A microtiter procedure for the quantitation of Bacillus cereus hemolysin is described.

We constructed a promoter-trap plasmid, pAD123, for Bacillus cereus. This plasmid contains a promoterless gene that encodes a mutant version of the green fluorescent protein, GFPmut3a, that is optimized for fluorescence-activated cell sorting [Cormack, B.P., Valdivia, R.H., Falkow, S., 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.]. The plasmid replicates...

The biosorption mechanism is an alternative for chemical precipitation and ultrafiltration which have been employed to treat heavy metal contamination with a limited success. In the present study, three species of Bacillus which were isolated from solar salterns were screened for their detoxification potential of the heavy metals, lead, chromium, and copper, by biosorption. Biosorption potentia...

several methods have been developed for gold exploration in the past, among which biological base method is known to be the most efficient with least expenses. this method can also be used for latent gold prospects exploration. in the present study, the possibility of applying bacillus cereus frequency in soil as a biological marker was investigated for the exploration of latent gold prospectin...

The structures of the two fengycins, lipopeptides from Bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. They show a close structural relationship to the plipastatins from Bacillus cereus differing only in the stereochemistry of the Tyr residues.

Two monoclonal antibodies (MAbs) against Bacillus cereus were produced. The MAbs (8D3 and 9B7) were selected by enzyme-linked immunosorbent assay for their reactivity with B. cereus vegetative cells. They reacted with B. cereus vegetative cells while failing to recognize B. cereus spores. Immunoblotting revealed that MAb 8D3 recognized a 22-kDa antigen, while MAb 9B7 recognized two antigens wit...

The ethalonic extract of the leaves of Jatropha tanjorensis Ellis & Saroja was tested for antimicrobial activities against gram positive - Bacillus cereus, Bacillus subtilis, staphylococcus aureus, gram negative E. coli and mycobacterium pheli.

We report here the genome sequences of two bacteriophages of the Bacillus cereus group, DirtyBetty and Kida. These bacteriophages are double-stranded DNA-containing Myoviridae isolated from soil samples using Bacillus thuringiensis subsp. kurstaki as their host bacteria.

The soil-related Bacillus and Paenibacillus species have increasingly been implicated in various human diseases. Nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of Bacillus anthracis and Bacillus cereus, little is known on their pathogenicity for humans. In this study, we evaluated the use of matrix-assisted laser desorptio...

Spores of Bacillus megaterium, Bacillus cereus, and Bacillus subtilis were found to exhibit intrinsic paramagnetic properties as a result of the accumulation of manganese ions. All three Bacillus species displayed strong yet distinctive magnetic properties arising from differences in manganese quantity and valency. Manganese ions were found to accumulate both within the spore core as well as be...