Background Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods...

In the management of bacterial infection, identification of the pathogen after growth remains essential, so that the most appropriate treatment can be proposed even before antibiotic susceptibility data are available. The strategy for most common pathogens requires Gram staining, simple tests (eg, determination of oxidase and catalase activity), and appropriate phenotypic tests that require com...

background assessment of resistance genes is imperative, as they become disseminated to bacterial flora in plants and to the indigenous bacterial community, and thus ultimately contributes to the clinical problems of antibiotic resistant pathogens. objectives the research was to assess the antibiotic characteristics and incidence of sul3 genes of stenotrophomonas maltophilia isolates recovered ...

background: classical bacteriological detection of brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. however, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. methods: panel of some reference strains belonging to t...

Medical devices are often associated with hospital-acquired infections, which place enormous strain on patients and the healthcare system as well as contributing to antimicrobial resistance. One possible avenue for the reduction of device-associated infections is the identification of bacteria-repellent polymer coatings for these devices, which would prevent bacterial binding at the initial att...

(13 September, 2012 – Hong Kong) Hong Kong Sanatorium & Hospital (HKSH) is the first private hospital in Hong Kong to introduce Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometer for clinical service. With the innovative methodology of analyzing protein components of microorganisms and comparing them to reference patterns in a database, MALDI-TOF reduces th...

BACKGROUND Broad-range 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) is used for detection and identification of bacterial pathogens in clinical specimens from patients with a high suspicion for infection. However, prospective studies addressing the impact and clinical value of broad-range bacterial 16S rRNA gene amplification for diagnosis of acute infectious diseases in nonsel...

fire blight, a bacterial disease caused by erwinia amylovora (burr.) winslow et al. 1920 is considered as a serious disease on pome fruits. during recent years, this disease has caused serious damages to the fruit growing industry in iran. precise and reliable methods for identification and detection of the pathogen can help in its early control, prevent the disease spread and help eliminate th...

background and objectives: hospital surfaces can serve as reservoirs of potential pathogenic bacteria.staff hands are the main source of bacterial transmission in hospital. the prevalence of bacteria harboring β– lactamase enzyme in staff hands and hospital surfaces, leads to spread of β–lactamase producing bacteria and ultimate increase of antibiotic resistance nosocomial infections. the aim o...

a3-year-old goat weighing 40 kg was presented to the largeanimal unit, university veterinary hospital, universiti putramalaysia with complains of in appetence, lateral recumbency,and weakness. physical examination findings were pyrexia,tachycardia, tachypnea, and pale mucous membrane. harsh andcrackles lung sound was noticed upon auscultation. the case wastentatively diagnosed as pneumonic past...