Seven agricultural wastes named wheat bran (WB), rice straw (RS), wheat straw (WS), corn cob (CC), sugarcane bagasse (SCB), potato peal (PP) and banana peal (BP) were used for isolation of bacterial strains and as substrate for cellulases (CMCase, FPase and avicelase) and xylanase production. Five of the isolated strains were thermophilic and alkaliphilic. These bacterial isolates were tested t...

An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96% similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y ...

Microbial degradation of petroleum hydrocarbons is one of the major practices in natural decontamination process. The present study investigated about the isolation of bacteria from crude oil contaminated site and gravimetric analysis of degradation in which, two bacterial isolates formed maximum clearing zone on mineral salt medium. Among these isolate S2 showed maximum growth (0.85mg/ml) and ...

Background: Magnetic separation using magnetic nanoparticles can be used as a simple method to isolate desulfurizing bacteria from a biphasic oil/water system. Objectives: Magnetite nanoparticles were applied to coat the surface of Rhodococcus erythropolis IGTS8 and Rhodococcus erythropolis FMF desulfurizing bacterial cells, and the viability and...

Different types of wounds samples were collected from 19 patients during March-April 2011 in Namakkal, Tamil Nadu, India. Four types of bacterial species were isolated and identified by selective culture medium and standard biochemical tests from the collected wound samples. Each wound samples showed one or more bacterial isolate, totally 78.9% of samples exhibited 24 isolates. Among them, Stap...

Molecular information about the bacterial composition of a coculture capable of sulfate reduction after exposure to oxic and microoxic conditions was used to identify and subsequently to isolate the components of the mixture in pure culture. PCR amplification of 16S ribosomal DNA fragments from the coculture, analyzed by denaturing gradient gel electrophoresis, resulted in two distinct 16S ribo...

Our objective was to investigate the concomitant production of protease and lipase by a bacterial strain. A promising bacterial strain was isolated from a food-processing industrial effluent, which can produce both protease and lipase. The isolate was characterized by sequencing the 16S rRNA gene. The PCR amplified gene was subjected to analysis by BLAST to ascertain the genetic relatedness of ...

A mixed-sequence synthetic oligonucleotide probe was used to isolate a clone containing the gene encoding the alpha subunit of bacterial luciferase from Vibrio harveyi and part of the gene coding for the beta subunit. DNA sequence analysis has allowed us to determine that the genes are closely linked on the bacterial chromosome and transcribed in the same direction. Comparison of the sequences ...

Bacterial Identification, first described by Bryant (1986), is a program that provides a novel way of enhancing the teaching of systematic bacteriology and numerical identification procedures. A student is assigned an unknown isolate from a list of bacteria. The student's objective is to identify the unknown isolate using the least number of tests from the set of tests available. Simultaneously...

Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A...