The Streptococcus Invasion Locus (Sil) was first described in Streptococcus pyogenes and Streptococcus pneumoniae, where it has been implicated in virulence. The two-component peptide signaling system consists of the SilA response regulator and SilB histidine kinase along with the SilCR signaling peptide and SilD/E export/processing proteins. The presence of an associated bacteriocin region sug...

The discovery of novel probiotic bacteria from free-ranging animals for the treatment inflammatory bowel disease in domestic pets is a unique approach. chloroform extraction gastrointestinal (GI) tract material was used to inactivate vegetative cells and select spore-forming bacteria. A bacterium identified as Paenibacillus sp. strain via small ribosomal RNA (16S) gene sequencing isolated GI gr...

bacteriocins are proteinaceous antibacterial compounds that exhibit bactericidal activity against species closely related to the producer strain. the aim of this research was to investigate the production of bacteriocin by bacillus spp. isolated from intestinal bacterial flora of the caspian frisian roach (rutilus frisii kutum). a bacteriocin produced by the bacterium bacillus cereus strain rf ...

Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance was isolated from a plasmid gene disruption library. The plasmid insertion conf...

BACKGROUND Our previous comparative genomic analysis of Staphylococcus equorum KS1039 with five S. equorum strains illuminated the genomic basis of its safety and salt tolerance. However, a comprehensive picture of the cellular components and metabolic pathways involved in the degradation of macromolecules and development of sensory properties has not been obtained for S. equorum. Therefore, in...

Lactic Acid Bacteria (LAB) were isolated from traditional sourdough samples, and functional properties of selected LAB species determined. Five distinct strains Limosilactobacillus fermentum LR1, Lacticaseibacillus rhamnosus LR2, Levilactobacillus senmaizukei CE37, Lactiplantibacillus plantarum CE48 reuteri KD44A identified following the genotypic discrimination hundred isolates. Presence bacte...

enterococcus and lactococcus are gram-positive cocci that often occur in pairs ( diplococci ) or short chains, and are difficult to differentiate from streptococci on physical characteristics alone. enterococcus faecium because have concern antibiotic resistant, consider difficult as probiotic. the results of an assay show that a probiotic e. faecium strain might be a potential recipient of van...

The high-affinity iron assimilation system of plasmid ColV-K30 was cloned on the vector plasmid pPlac. Plasmid pABN1 was isolated by means of sensitivity to cloacin, a bacteriocin using the same outer membrane receptor as ferric aerobactin. Restriction maps were determined for this plasmid and for a subclone, pABN5. Plasmid pABN1 codes for the complete gene complex, whereas plasmid pABN5 encode...

The bacteriocinogenic plasmid Clo DF13 is present in Escherichia coli to the extent of 10 copies per cell. A complex of Clo DF13 plasmid deoxyribonucleic acid (DNA) and protein can be isolated from cells. Treatment of the complex with ionic detergents or proteases dissociates the complex but does not convert any supercoiled Clo DF13 DNA to the open circular form, indicating that this complex is...

Listeria monocytogenes is a foodborne pathogen associated with fermented products. The aim of this study was to characterize anti-listerial bacteriocin produced by lactic acid bacteria (LAB) isolated from Cambodian fermented foods. Four LAB isolates of confirmed bacteriocin production were isolated from fermented fish and shrimp (pha ak trey, pha ak kampus). These isolates demonstrated antimicr...