Expression in vitro with the recombinant baculovirus expression system showed correct biosynthesis and post-translational processing of "wild-type' bovine opsin with regard to translocation, glycosylation, palmitoylation and targeting. However, several of these processes were severely affected by point mutations. From the overall results of 16 mutants reported here, four groups were distinguish...

The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and ...

Protein phosphatase 2A (PP2A) is an enzyme useful for detecting several natural toxins represented by okadaic acid and microcystins. We found that the production of the recombinant human PP2A catalytic subunit (rhPP2Ac) in High Five insect cells could markedly increase when the cells were cultured at 19 °C instead of 27 °C used under conventional conditions. The yield and purity of the enzyme i...

The g -tubulin ring complex ( g TuRC) is important for microtubule nucleation from the centrosome. In addition to g -tubulin, the Drosophila g TuRC contains at least six subunits, three of which [ Drosophila gamma ring proteins (Dgrips) 75/d75p, 84, and 91] have been characterized previously. Dgrips84 and 91 are present in both the small g -tubulin complex ( g TuSC) and the g TuRC, while the re...

The baculovirus expression vector system has been commonly employed as a rapid and inexpensive approach for recombinant protein production in insect cells (1, 2). Despite the wide applications, baculovirus infection results in insect cell death and lysis in a few days post-infection, which can lead to suboptimal protein processing late in the infection phase. In addition, some proteins that are...

Recombinant protein expression using the Baculovirus Expression System is widely used for the production of therapeutics, diagnostics, and reagents. The current “gold standard” for Baculovirus titration, the plaque assay, is labor intensive and time consuming. The realtime PCR assay has the advantage of speed and accuracy. We determined Baculovirus tire by a novel quantitative PCR (qPCR) method...