To test the hypothesis that glycerol would concomitantly affect sperm membrane structure and the function of the intact cells, boar semen (4 ejaculates from 4 boars) was cryopreserved in an egg yolk extender with 0%, 2%, 4%, or 8% glycerol in 0.5-mL straws using previously derived optimal cooling and thawing rates. Increasing glycerol concentrations increased spermatozoal progressive motility i...

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg mL(-1) sodium selenite and the sperm motility a...

In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreserva...

Northern pintail duck semen and sperm traits were characterized, and the fertility of cold-stored spermatozoa was investigated using artificial insemination. Excellent quality ejaculates containing high proportions of motile spermatozoa were collected from drakes within 20 s by a massage technique. Semen was collected in Beltsville poultry semen extender, pooled and cold-stored (4 degrees C) fo...

We define extender sequences, generalizing measure sequences of Radin forcing. Using the extender sequences, we show how to combine the Gitik-Magidor forcing for adding many Prikry sequenes with Radin forcing. We show that this forcing satisfies a Prikry like condition destroys no cardinals, and has a kind of properness. Depending on the large cardinals we start with this forcing can blow the p...

Aim of this study is to evaluate the effect adding different levels Zinc and Selenium on extender as antioxidants improve Friesian-bull semen quality after cryopreservation. Semen was collected, pooled, extended with five treatment extenders, including control without (E1), 0.3 mg/100ml Zn (E2), 0.6 (E3), 0.2 Se (E4), 0.5 (E5). evaluated for visual parameters dilution, equilibration, thawing. A...

The aim of the present study was to evaluate cryoprotective effect trehalose on stallion semen quality like progressive sperm motility, viability, acrosome integrity, membrane integrity. A total 42 ejaculates from six Marwari (seven ejaculate each) were used. Immediately after gross evaluation, filtered into a pre warm, graduated measuring bottle get gel free and it diluted with primary extende...

MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored watersoluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studi...

An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5 ̊C, 25 ̊C and 41 ̊C) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil extend...