نتایج جستجو برای: bead array

تعداد نتایج: 140843  

Journal: :Clinical chemistry 2011
Athina Markou Areti Strati Nikos Malamos Vasilis Georgoulias Evi S Lianidou

BACKGROUND Molecular characterization of circulating tumor cells (CTCs) is crucial to identify novel diagnostic and therapeutic targets for individualized therapies. We developed a multiplexed PCR-coupled liquid bead array to detect the expression of multiple genes in CTCs. METHODS mRNA isolated from immunomagnetically enriched CTCs was subjected to multiplex PCR for KRT19 (keratin 19; also k...

2016
Subodh Kumar Nidhi Bhardwaj Surbhi Khurana Amit Gupta Kapil Dev Soni Richa Aggrawal Purva Mathur

AIM OF STUDY Ventilator-associated pneumonia (VAP) is a common cause of mortality in trauma patients admitted to Intensive Care Units. The outcome of such patients may be dependent on local host immune response, which may be best reflected in studies using bronchoalveolar lavage (BAL) fluid. The present study was conducted to ascertain the cytokine profile of BAL using the cytometric bead array...

Journal: :Bioinformatics 2009
E. Andres Houseman Brock C. Christensen Margaret R. Karagas Margaret R. Wrensch Heather H. Nelson Joseph Wiemels Shichun Zheng John K. Wiencke Karl T. Kelsey Carmen J. Marsit

MOTIVATION Integration of various genome-scale measures of molecular alterations is of great interest to researchers aiming to better define disease processes or identify novel targets with clinical utility. Particularly important in cancer are measures of gene copy number DNA methylation. However, copy number variation may bias the measurement of DNA methylation. To investigate possible bias, ...

Journal: :Journal of clinical microbiology 2007
Collette Fitzgerald Marcus Collins Susan van Duyne Matthew Mikoleit Teresa Brown Patricia Fields

We report the development and evaluation of a Salmonella O-group-specific Bio-Plex assay to detect the six most common serogroups in the United States (B, C(1), C(2), D, E, and O13) plus serotype Paratyphi A. The assay is based on rfb gene targets directly involved in O-antigen biosynthesis; it can be completed 45 min post-PCR amplification. The assay correctly and specifically identified 362 o...

Journal: :Clinical chemistry 2003
Attila Tárnok Jörg Hambsch Roy Chen Rudi Varro

Infections and sepsis are among the most common reasons for neonatal morbidity and mortality. Early diagnosis is difficult because clinical presentation is highly variable and signs are often subtle and common to a variety of conditions. Among the proposed early indicators of infection and sepsis are serum concentrations of interleukin (IL)-6, IL-8, and IL-10. It is believed that IL-8 is a sens...

Journal: :Molecular vision 2006
Richard Maier Martin Weger Eva-Maria Haller-Schober Yosuf El-Shabrawi Anna Theisl Alfred Barth Reingard Aigner Anton Haas

PURPOSE This study was carried out to compare cytometric bead array (CBA) technology with conventional enzyme linked immunosorbent assay (ELISA) for the measurement of both vitreous and serum concentrations of interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), and angiogenin (ANG) in diabetic and non-diabetic patients. METHODS Measurement of vitreous and serum concentrations of ...

2015
Yi-Chen Yang Der-Yuan Wang Hwei-Fang Cheng Eric Y Chuang Mong-Hsun Tsai

The genotyping of the hepatitis C virus (HCV) plays an important role in the treatment of HCV because genotype determination has recently been incorporated into the treatment guidelines for HCV infections. Most current genotyping methods are unable to detect mixed genotypes from two or more HCV infections. We therefore developed a multiplex genotyping assay to determine HCV genotypes using a be...

Bhaskar Venkata Kameswara Subrahmanya Lakkakula Kumarasamy Peria Priyanka Venugopal Solomon Franklin Durairaj Paul Suresh Ranga Rao Vamsi Lavu Vettriselvi Venkatesan

Background: Cytokines are suggested to play a role in periodontitis. Objective: To determine and compare the levels of Interleukin-1 beta (IL-1β) and Tumor necrosis factor alpha (TNF-α) in gingival crevicular fluid (GCF) samples amongst healthy individuals and  those with chronic periodontitis. Further to compare the GCF cytokine levels in three genotype classes defined by the respective gene p...

2011
Stijn van Pelt Roy Derks Marco Matteucci Mikkel Fougt Hansen Andreas Dietzel

A new concept for the manipulation of superparamagnetic beads inside a microfluidic chip is presented in this paper. The concept allows for bead actuation orthogonal to the flow direction inside a microchannel. Basic manipulation functionalities were studied by means of finite element simulations and results were oval-shaped steady state oscillations with bead velocities up to 500 μm/s. The wid...

2007
Sergey Kasparov Sanjay Tyagi Caitlin Smith Anna Karina Busch Thomas Litman Martin Schuerenberg Christine Luebbert Sören-Oliver Deininger Ralf Ketterlinus

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