サステイナブルな下水処理系の構築に向けて、産業排水等に含まれる有機成分を基質とし、余剰汚泥をバイオ触媒として用いて、化成品等の有価物を生産する戦略が提案できる。すなわち、下水処理場をバイオリファイナリーに進化させる構想である。この構想を実現するうえで核となる技術として、余剰汚泥によるバイオプラスチックPHA（ポリヒドロキシアルカン酸）の生産に関しての技術開発を進めている。下水処理場由来の余剰汚泥は特に馴致せずとも、産業排水等に含まれる揮発性脂肪酸や糖類などの比較的広範な基質からPHAをある程度生産する能力を有していることが示されている。また、ADD法（aerobic dynamic discharge process）により、余剰汚泥中のPHA蓄積微生物を集積することで、2日間程度の極めて短期間にPHA生産効率を飛躍的に向上し得ることできる。これらの研究開発の成果を活用することで、下...

In the present paper, the biodesulfurization of simulated light fuel oil (i.e., dibenzothiophene (DBT) in dodecane) and untreated kerosene with a high total sulfur content has been studied by a native isolated bacterium named Bacillus cereus HN. The influences of various parameters such as the reaction temperature (T), biocatalyst cell density, oil phase fraction (OFP), and initial DBT concentr...

Albumin emerged as a biocatalyst in 1980 and the continuing interest in this protein is proved by numerous papers. The use of albumin was initially confined to the field of asymmetric oxidations and reductions, but more recently it has found a broader application to chemical reactions such as additions, condensations and eliminations. This review reports the main applications of albumin in orga...

Biocatalyst mediated regio- and stereo-selective hydroxylation and epoxidation on (Z)-α-santalol were achieved for the first time, using a fungal strain Mucor piriformis. Four novel metabolites were characterized as 10,11-cis-β-epoxy-α-santalol, 5α-hydroxy-(Z)-α-santalol, 10,11-dihydroxy-α-santalol and 5α-hydroxy-10,11-cis-β-epoxy-α-santalol. Using Amano PS lipase from Burkholderia cepacia, α- ...

A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfid...

in the present paper, the biodesulfurization of simulated light fuel oil (i.e., dibenzothiophene (dbt) in dodecane) and untreated kerosene with a high total sulfur content has been studied by a native isolated bacterium named bacillus cereus hn. the influences of various parameters such as the reaction temperature (t), biocatalyst cell density, oil phase fraction (ofp), and initial dbt concentr...

The process of jatropha oil transesterification was studied for biodiesel production. Rhizomucor miehei lipase (RML) immobilized on 3-aminopropyltriethoxysilane functionalized magnetic Fe3O4 employed as a biocatalyst. confirmed by scanning electron microcopy (SEM), Fourier transform infrared spectra (FT-IR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and confocal las...

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due its biodegradability and less toxic level. Biodiesel can synthesized through non-alcohol route by substituting alcohol with methyl acetate using lipase enzyme as Biocatalyst, which may give by-product during reaction. In this research, from Bacillus subtilis ha...