نتایج جستجو برای: bioluminescence emission spectrum
تعداد نتایج: 396029 فیلتر نتایج به سال:
Novel methods are required to investigate the complexity of microRNA (miRNA) biology and particularly their dynamic regulation under physiopathological conditions. Herein, a novel plasmid-based RNAi-Inducible Luciferase Expression System (RILES) was engineered to monitor the activity of endogenous RNAi machinery. When RILES is transfected in a target cell, the miRNA of interest suppresses the e...
PURPOSE Bacteria-based tumor-targeted therapy is a modality of growing interest in anticancer strategies. Imaging bacteria specifically targeting and replicating within tumors using radiotracer techniques and optical imaging can provide confirmation of successful colonization of malignant tissue. EXPERIMENTAL DESIGN The uptake of radiolabeled pyrimidine nucleoside analogues and [18F]FDG by Es...
The luxA, B, C, D, and E genes from Photorhabdus luminescens were cloned and functionally expressed in Saccharomyces cerevisiae to construct a bacterial lux-based yeast bioreporter capable of autonomous bioluminescence emission. The bioreporter was engineered using a series of pBEVY yeast expression vectors that allowed for bi-directional constitutive or inducible expression of the individual l...
Thermoluminescence TL materials LiF(Mg,Cu2+) and LiF(Cu2+) were produced in powder. Their 3D emission spectra were investigated and compared to the commercially available LiF(Mg,Cu2+,P) and (LiF(Mg,Ti) TL materials. Emission spectrum of LiF(Mg,Cu2+) represents a peak maximum at 450 nm with a wide shoulder ranging from 520 nm to infrared area. Emission peak of LiF(Cu2+) is situated at 380 nm and...
Fish do it. Dolphins do it. Swimmers do it. Surface ships do it. In fact, the last German U-boat detected in the Great War was sunk because it did it. What they all do is create flow fields energetic enough to create bioluminescent "footprints." Because bioluminescent organisms are ubiquitous (particularly dinoflagellates, common members of marine plankton) flow-stimulated light emission repres...
The blue glow of the mucus from Chaetopterus involves a photoprotein, iron and flavins. Identity and respective role of these components remain, however, largely unresolved today, likely because of viscosity issues and inhibition of this system by oxidizers conventionally used to track bioluminescence activity. Here, we used gentle centrifugation to obtain a mucus supernatant showing no inhibit...
fabrication and characterization of visible light active fe-tio2 nanocomposites as nanophotocatalyst
in this research fe-tio2 nanocomposites with different molar ratios of fe/ti were prepared as nano-photocatalyst using a modified sol-gel process at ambient temperature. crystallographic properties of nanocomposites were characterized by x-ray diffraction (xrd). surface morphology and mean particle size of nanocomposites were specified by field emission scanning electron microscopy (fesem) and ...
Streptococcus pyogenes infection of the nasopharynx represents a key step in the pathogenic cycle of this organism and a major focus for vaccine development, requiring robust models to facilitate the screening of potentially protective antigens. One antigen that may be an important target for vaccination is the chemokine protease, SpyCEP, which is cell surface-associated and plays a role in pat...
MOTIVATION Bacterial luciferases are heterodimeric enzymes that catalyze a chemical reaction, so called bioluminescence, which causes light emission in bacteria. Bioluminescence is vastly used as a reporter system in research tools and commercial developments. However, the details of the mechanisms that stabilize and transform the reaction intermediates as well as differences in the enzymatic k...
Inertial bioluminescence rhythms at the Capo Passero (KM3NeT-Italia) site, Central Mediterranean Sea
In the deep sea, the sense of time is dependent on geophysical fluctuations, such as internal tides and atmospheric-related inertial currents, rather than day-night rhythms. Deep-sea neutrino telescopes instrumented with light detecting Photo-Multiplier Tubes (PMT) can be used to describe the synchronization of bioluminescent activity of abyssopelagic organisms with hydrodynamic cycles. PMT rea...
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