The cross-linking agent ethylene glycol-bis(succinimidyl succinate) was used to covalently link 125I-labeled gastrin releasing peptide (125I-GRP) to an Mr 75,000-85,000 surface protein in Swiss 3T3 cells that displays many characteristics of a specific receptor for peptides of the bombesin family. This protein was not present in other cell lines which do not exhibit receptors for bombesin-like ...

Female Syrian golden hamsters with /V-nitrosobis(2-oxopropyl)amineinduced pancreatic cancers were treated for 2 months with new pseudononapeptide bombesin receptor antagonist |D-Tpi6,Leu'V(CH2NH)Leul4)bombesin(6-14XRC-3095), administered s.c. with implanted os motic minipumps releasing 20 «ig/dayof the analogue. The results were compared to those obtained by treatment with somatostatin analogu...

Bombesin-like neuropeptides, including mammalian gastrin-releasing peptide (GRP), are potent mitogens for Swiss 3T3 cells. In this study, we have characterized the bombesin receptor in membrane preparations from these cells. Addition of Mg2+ during cell homogenization was essential to preserve 125I-GRP binding activity in the resulting membrane preparation. The effect of Mg2+ was concentration ...

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introduction: it has been shown that some primary human tumors and their metastases, including prostate and breast tumors, over-express gastrin-releasing peptide (grp) receptors. bombesin is a neuropeptide with a high affinity for these grp receptors. the purpose of this study was to prepare and evaluate the characteristics of a new freeze-dried kit, [6-hydrazinopyridine-3-carboxylic acid (hyni...

Recent immunohistochemical findings have indicated the presence of gastrin-releasing peptide in normal and pathological human lungs. Gastrin-releasing peptide is a 27-amino acid peptide isolated from porcine gut which bears considerable carboxyterminal homology with bombesin. We have characterized the gastrin-releasing peptide-like peptides present in a human malignant lung carcinoid tumor by g...