By accurately measuring the difference infrared spectrum of Cot-equilibrated bovine carbonic anhydrase against the ethoxzolamideor azide-inhibited enzyme, we have been able to obtain the absorption maximum at 2341 cm-l that is due to the asymmetric stretching of the CO2 molecule loosely bound to a hydrophobic surface at the active site of the enzyme. COz dissolved in water absorbs at 2343.5 cm-...

The carbonic anhydrase (CA: Carbonate hydrolyase: E.C.4.2.1.1) content from the leaves and flowers of mature yarrow (Achillea millefolium), a plant believed to possess healing qualities, was purified and characterized. The purification levels were 35.58 fold in the leaves and 40.21 fold in the flowers. The optimum temperatures were 25C and 40C for the leaves and flowers, respectively, and optim...

The distribution and biochemical diversity of neuroglial cells in the avian optic tectum were analyzed using immunohistochemical techniques. Polyclonal and monoclonal antibodies were employed to define the distributions and localizations of four proteins that are considered to be glial-specific in the mammalian central nervous system: glutamine synthetase, carbonic anhydrase-C, glial fibrillary...

allura red (ar) is a widely used colorant in food industry, but there is debate on its potential security risk. in this study, in vitro inhibitory properties of the dye against carbonic anhydrase (ca) were evaluated. the esterase activity of purified ca decreased in the presence of ar, in a dose-dependent manner. regarding literature review and observed results, this preliminary study may provi...

PURPOSE To report the combination cocktail of strong steroid, non-steroidal anti-inflammatory drug (NSAID), and carbonic anhydrase inhibitor drops for treatment of cystoid macular edema. METHODS This is a retrospective case series of patients with cystoid macular edema managed with a topical combination of strong steroid (difluprednate), NSAID, and carbonic anhydrase inhibitor drops. The pati...

in this paper, we used quantum chemical approach to shed light on the catalytic mechanism of γ-carbonic anhydrase (γ-ca) to convert carbon dioxide to bicarbonate ion. density functional theory (dft) using b3lyp and ub3lyp functional and three split-valance including 6-31g*, 6-311g** and 6-311++g** basis sets were used to calculate the details of electronic structure and electronic energy of act...

binary logistic regression (blr) has been developed as non-linear models to establish quantitative structure- activity relationships (qsar) between structural descriptors and biochemical activity of carbonic anhydrase inhibitors. using a training set consisted of 21 compounds with known ki values, the model was trained and tested to solve two-class problems as active or inactive on the basis of...

It was previously reported that magnesium ion inhibited carbonic anhydrase (Bamberger and Avron 1975 Plant Physiol 56: 481-485). Studies with partially purified carbonic anhydrase from spinach (Spinacia oleracea L.) chloroplasts show that the effect was the result of the chloride counterion and not the magnesium ion. Enzyme activity was reduced 50% upon addition of 3 to 10 millimolar MgCl(2) or...