Amine-functionalized mesoporous SBA-15 silica loaded with bovine serum albumin (BSA) has been successfully encapsulated with a thin layer coating of poly(acrylic acid) PAA, with the entrapped BSA being released from the PAA-encapsulated SBA-15 at the higher pH value of 7.4 rather than at the lower pH value of 1.2. This novel drug delivery system has a potential application in the release of pro...

This work reports the self-assembly of anion-exchangeable vesicles from an amphiphilic hyperbranched polymeric ionic liquid (HBPIL). By a simple one-step anion exchange with methyl orange, the obtained HBPILs could self-assemble into pH-indicative and colorful vesicles in water with color changes directly visible to the naked eye in response to solution pH. In addition, by another step of anion...

The interaction between bovine serum albumin (BSA) and atazanvir sulphate (AS) has been investigated under the physiological pH 7.4 condition using fluoresence spectroscopy. The binding constant, number of binding site, thermodynamic parameters such as ∆G, ∆H, ∆S and nature of binding forces between BSA-AS have been obtained from the steady state fluorescence quenching of BSA by AS. The static ...

A new direct method has been developed to determine protein-drug binding based on hollow fiber membrane solvent microextraction. Hollow-fiber membrane solvent microextraction coupled with high-performance chromatography with UV detection was employed to evaluate the binding characteristics of drugs to bovine serum albumin (BSA) and blood serum. It was found that the BSA and matrix in the blood ...

Molecular imprinted polymers (MIP) have key-lock pattern binding properties specific to the size and shape of target molecules. In this study, we prepared detection platforms based on a molecularly surface plasmon resonance (SPR) sensor that can detect bovine serum albumin (BSA) sensitively, selectively, quickly, in real time. The polymeric film SPR by molecular imprinting method was obtained s...

adsorption of bovine serum albumin (bsa) onto calcium hydroxyl apatite (ha), ca10po4(oh)2, has been studied by ultra violet (uv) spectroscopy. the adsorption isotherms of bsa onto the ha surface at 291.7 k and 303.2 k were satisfactorily presented by langmuir equation and the evaluated parameters are reported. the specific surface area of ha has been measured by the bet method. the obtained val...

Dithiocarbamates, with their high lipophilic character and good chelating properties, could provide stabile transition metal complexes enable these metal-based drugs to reach biological targets. Palladium(II) gold(III) complexes, due its structural similarity platinum(II)compounds, be competitive candidates for implementing developing new pharmacological agents. Herein, novel complex triammoniu...

Previously we have shown that (-)-epigallocatechin gallate (EGCG) can induce nonapoptotic cell death in human hepatoma HepG2 cells only under serum-free condition. However, the underlying mechanism for serum in determining the cell fate remains to be answered. The effects of fetal bovine serum (FBS) and its major component bovine serum albumin (BSA) on EGCG-induced cell death were investigated ...

Kinetic models are essential for describing how molecules interact in a variety of biochemical processes. The estimation model’s kinetic parameters by experiment enables researchers to understand pathogens, such as viruses, with other entities like antibodies and trial drugs. In this work, we report simple proof-of-principle that uses quantum sensing techniques give more precise than is possibl...

Membrane fouling by the high protein concentrations used in biopharmaceutical industry remains poorly understood. In this study, feed of up to 10,000 ppm bovine serum albumin (BSA) were investigated with three polymeric membranes, two which had same molecular weight cut-off (namely, PES100 and PSF100), membrane material PES10). A crossflow ultrafiltration (UF) setup equipped electrical impedanc...