There are strong indications that oxidative phosphorylation processes are involved in the activation (or “priming, ” “sparking” (1)) of enzymatic fatty acid oxidation (l-5). Two major points of evidence exist: first, it is known that simultaneous oxidation of intermediates of the Krebs tricarboxylic acid cycle under conditions in which oxidative phosphorylation may be observed is required to ac...

The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed mo...

staff Coomassie Brilliant Blau G250. J Clin Chem Cm Biochem 1981;19:203-8. 5. Peterson GL Determination of total protein.Methods Enzymol 1983;91:95-119. 6. SedmakJJ,GroesbergSE.A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250. Anal Biochem 1977;79:544-52. 7. JohnsonA, Lott JA. Standardization of the Coomassie Blue method forcerebrospinal fluid proteins. Cl...

When living cells of Nitella are exposed to an acetate buffer solution until the pH value of the sap is decreased and subsequently placed in a solution of brilliant cresyl blue, the rate of penetration of dye into the vacuole is found to decrease in the majority of cases, and increase in other cases, as compared with the control cells which are transferred to the dye solution directly from tap ...

The Piedmont enjoys a highly regarded reputation for its historic sites and its world-class preservation practices. The well-preserved homes of presidents and extraordinary examples of architecture, known worldwide, dominate our landscape. We see daily the many ways in which preservation work enriches the lives of people who live in, work in, and visit our community. Numerous residents in the P...

Expelling of dyestuff into water resource system causes major thread to the environment. Adsorption is the cost effective and potential method to remove the dyes from the effluents. Therefore, an attempt was made to study the adsorption of dyestuff (Methylene Blue (MB), Bromophenol Blue (BPB) and Coomassie Brilliant Blue (CBB)) by α-chitin nanoparticles (CNP) prepared from Penaeus monodon (Fabr...

The relationship between pentose phosphate pathway (PPP) activity in cumulus-oocyte complexes (COCs) and oxidative and mitochondrial activity in bovine oocytes was evaluated with the aim of analysing the impact of two inhibitors (NADPH and 6-aminonicotinamide (6-AN)) and a stimulator (NADP) of the key enzymes of the PPP on the maturation rate, oxidative and mitochondrial activity and the mitoch...

Although differences in the quality of oocytes derived from young gilts and adult sows are well documented, evidence concerning gametes of pre-pubertal and cycling gilts is scarce and inconsistent. The aim of this work was to establish whether sexual maturity of gilts affects the quality of their oocytes with the use of the brilliant cresyl blue (BCB) test, oocyte diameter and apoptosis. Ovaria...

Increasing environmental pollution is connected with broad applications of dyes and imperfection of dyeing technology. Decolourization of triphenylmethane brilliant green and disazo Evans blue by bacterial and fungal strains and toxicity (phyto- and zootoxicity) of degradation by-products were investigated. Influence of incubation method on dyes removal was evaluated (static, semi-static, shake...