Small conductance Ca2+-activated K+ (SKCa) channels comprise a widely distributed but relatively little studied class of K+ channels.1 Selective blockade of SKCa channels may find application in the therapy of myotonic muscular dystrophy,2 gastrointestinal dysmotilities (Dunn, P. M.; Jenkinson, D. H., unpublished results), disorders of memory,3 narcolepsy,4 and alcohol abuse.5 Furthermore, SKCa...

Mono- and polynuclear complexes containing 3-(1,10-phenanthrolin-2-yl)-1-(pyridin-2-ylmethyl)imidazolylidene (L), [NiL(2)](PF(6))(2) (2), [CoL(2)](PF(6))(3) (3), [PtLCl](PF(6)) (4), [PdAgL(2)](PF(6))(3) (5), [PdCuL(2)](PF(6))(3) (6), [Pd(2)L(2)Cl(2)](PF(6))(2) (7), and [Pd(3)L(2)Cl(4)](PF(6))(2) (8) have been prepared and fully characterized by NMR, ESI-MS spectroscopy, and X-ray crystallograph...

A multifunctional reagent based on a coumarin scaffold was developed for derivatization of naive RNA. The alkylating agent N3BC [7-azido-4-(bromomethyl)coumarin], obtained by Pechmann condensation, is selective for uridine. N3BC and its RNA conjugates are pre-fluorophores which permits controlled modular and stepwise RNA derivatization. The success of RNA alkylation by N3BC can be monitored by ...

The success of all crosslinking reagents is idiosyncratic. In crosslinking experiments with bBBr, the bromomethyl group that remains in an activated monomer is a target for both intramolecular and intermolecular nucleophiles. Fortunately, thiolate is by far the most reactive biomolecular nucleophile toward bBBr (2). Indeed, the high specificity of bBBr for reaction with cysteine residues may ma...

K-region epoxides of benz[a] anthracene, dibenz[a,h] anthracene, and 3-methylcholanthrene have been found to be toxic and more active in producing malignant transformation of cells derived from mouse prostate than their respective parent hydrocarbons and K-region dihydrodiols and phenols. The data support the view that metabolism of these polycyclic hydrocarbons is a prerequisite for their biol...

A microwave-assisted, fluorous synthetic route to 3-amino-1,2-benzisoxazoles and 4-amino-1H-2,3benzoxazines has been developed. The strategy comprises linking the respective 2-fluorobenzonitrile or 2-(bromomethyl)benzonitrile to a fluorous oxime tag to give an aryloxime intermediate which then undergoes cyclization with concomitant cleavage of the substrate-tag in acidic conditions to provide t...