The 97 subje ts studied have been grouped in 7 classes (table 1): class 1, 30 normal subjects, 16 to 48 years old, without clinical signs of atherosclerosis; class 2, 27 older subjects (aged 36 to 93) with clinical signs of atherosclerosis; classes 3 to 7, 40 diabetic subjects, 10 being without clinical signs of vascular degeneration, the others presenting various vascular complications. The la...

This study describes the male reproductive cycle of Sibynomorphus mikanii from southeastern Brazil considering macroscopic and microscopic variables. Spermatogenesis occurs during spring-summer (September-December) and spermiogenesis or maturation occurs in summer (December-February). The length and width of the kidney, the tubular diameter, and the epithelium height of the sexual segment of th...

Two simple, rapid and accurate methods for the determination of bupropion hydrochloride (BUP) in pure and in pharmaceutical preparations are described. Both methods are based on the measurement of the chloride of its hydrochloride. In the titrimetric method, the chloride content of bupropion hydrochloride is determined by titrating with mercury(II)nitrate using diphenylcarbazone–bromophenol blu...

Unified schedule for multiple parallel solid-phase synthesis of so-called "difficult" peptides on polypropylene pins was developed. Increase in the efficiency of 9-fluorenyl(methoxycarbonyl) N-terminal amino-protecting group removal was shown to have a greater influence on the accuracy of the "difficult" peptide synthesis than the use of more efficient amino acid coupling reagents such as amini...

Three simple new visible spectrophotometric methods has been developed for determination of Losartan potassium and Omeprazole, in single component pharmaceutical dosage forms. The methods were based on the formation of ion-pair complexes of the drug with dye Bromocresol purple(BCP) and Bromophenol blue(BPB), in acidic buffer solutions followed by their extraction in chloroform. Experiments were...

Two novel vanadium(III) complexes: V(dipic)(Hbdc)(H2O)2 (1) and [V2(dipic)2(H2btec)(H2O)4]·2H2O (2) (H2dipic = 2,6-pyridinedicarboxylic acid, H2bdc = 1,3-benzene-dicarboxylic acid, H4btec = 1,2,4,5-benzenetetracarboxylic acid) are synthesized by the reaction of V2(SO4)3, 2,6-pyridinedicarboxylic acid and 1,3-benzene-dicarboxylic acid (for 1) or 1,2,4,5-benzenetetracarboxylic acid (for 2) under ...

Screening GenBank indicated that an esterase from Pseudomonas fluorescens had high sequence similarity with bacterial non-haem haloperoxides. However, this homology was limited to two distinct domains of the published esterase sequence. As errors in the published sequence were suspected, the esterase gene was sequenced again. The revised sequence displayed between 40 and 50% identical amino aci...

Because of its simplicity, PCR performed directly from bacterial colonies on agar plates has been extensively used as a first step in the identification of recombinant clones (1–3,6,7). Instead of making DNA minipreps and restriction digestion from several putative recombinant colonies, colony PCR allows the identification of the positive clones, and so reduces these procedures to one clone per...