Pollen viability and its germination capability for fruit set in are almond and peach essential. To optimize the pollen culture medium of almond and peach and selection of best medium, the present study was carried out with 48 different culture media containing different compositions including diverse levels of boric acid (0 and 100mg/1), calcium nitrate (0,150and300mg/l،), magnesium sulphate (...

Trypanosoma danilewskyi strain F Cc-1 was isolated from the blood of a laboratory infected juvenile common carp. Trypanosomes (blood stream forms) were first adapted to culture conditions in Tobie’s medium. Ten sterilized glass culture tubes each containing 2 ml of Tobie’s medium solidified in slants and covered with 1 ml Locke-Krebs overlay, were inoculated with 0.1 ml of infected blood contai...

Pulmonary surfactant is a developmentally and hormonally regulated lipoprotein synthesized exclusively in alveolar type II cells. Surfactant protein-A (SP-A) gene transcription in human fetal lung in culture is stimulated by glucocorticoids and cAMP; cAMP also enhances the rate of type II cell differentiation. The CCAAT/enhancer-binding protein (C/EBP) family of transcription factors serves an ...

  A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...

Microorganisms can produce a wide range of bio-based chemicals that be used in various industrial applications as molecules interest. In the present work, an analysis power production by pure culture, co-culture, and sequential culture was performed. this study, both mono-culture co-culture strategies Actinobacillus succinogenes with Saccharomyces cerevisiae carbon sources to succinic acid usin...

chromagar candida is a new chromogenic culture medium that is used for the isolation and direct identification of some of the most clinically important yeast pathogens on the basis of colony colour. the aim of the study was to isolate and identify candida species from the oral cavity with chromagar candida. samples (158) from 68 orthodontic patients wearing upper removable orthodontic appliance...

the incidence of candidemia due to the uncommon non-albicans candida species appears to be increasing, and certain species such as candida (c.) membranaefaciens have been reported in some clinical researches. vascular catheters are considered the likely culprit for the sudden emergence of hospital-acquired candidemia. the identification of c. membranaefaciens can be problematic in clinical prac...

AIM To assess the ability of commercial blood culture systems to maintain leptospires. METHODS Nine different commercial blood culture bottles were compared for their ability to maintain four leptospiral strains at two temperatures, 30 degrees C and 37 degrees C. Bottles were subcultured at 48 hours, and one, two, three, and four week intervals and examined microscopically for the presence of...

We aimed to evaluate the stability of the Chlamydia trachomatis multi locus VNTR analysis (MLVA-ompA) and multi sequence typing (MST) systems through multiple passages in tissue culture. Firstly, we analyzed the stability of these markers through adaptation of C. trachomatis to tissue culture and secondly, we examined the stability of a four-locus MLVA-ompA and a five-locus MST system after mul...

The goal of this study was to determine the time to detection of Candida species isolates using the two most commonly used automated blood culture systems, and to evaluate rapid, widely available methods for the presumptive identification of C. albicans. Candidemia models of eight commonly detected Candida species were prepared using ATCC standards. The times to detection were evaluated using t...