We describe a one-day micro-scale procedure for determining the total lipid profile. Only 0.55 mL of plasma is needed for complete quantification of total cholesterol (TC), triglyceride (TG), and all lipoproteins. After precipitation with dextran sulfate and magnesium, the high-density lipoprotein (HDL) fraction was separated by centrifugation in an Eppendorf microcentrifuge. Very-low-density l...

This study investigates the effect of altered lipoprotein free cholesterol (FC) content on the transfer of cholesteryl ester (CE) and triglyceride (TG) from very low- (VLDL), low- (LDL), and high-(HDL) density lipoproteins by the plasma-derived lipid transfer protein (LTP). The FC content of VLDL and HDL was selectively altered by incubating these lipoproteins with FC/phospholipid dispersions o...

The major plasma lipids, cholesteryl ester, triacylglycerol, cholesterol and phospholipid, are insoluble in water. They are transported in the circulation as lipoproteins-metabolically diverse macromolecules in which the various lipids are associated with specific polypeptides (the apoproteins). There are four main lipoprotein classes, defined by their size and by the density range within which...

References 1. Levy Y, Aviram M, Spira G, Tatarsky I, Brook GJ, Carter A. Plasma cholesterol concentration and extra lipid band in monoclonal gammopathies. Postgrad Med J 1984;60:449–53. 2. Kadri N, Douville P, Lachance P. Monoclonal paraprotein may interfere with the Roche Direct HDL-C Plus Assay. Clin Chem 2002;48:964. 3. C-C chemistry participant summary report, surveys 2002. Northfield, IL: ...

AIM To determine a potential relationship between serum undercarboxylated (ucOC) concentration and cardiovascular risk factors in type 2 diabetes (T2D) patients and healthy subjects (HS). METHODS A cross-sectional study was conducted on 140 subjects classified into two groups, 70 with T2D and 70 HS. Medical history and physical examination with anthropometric measurements were obtained from a...

Previously, we have shown that lipid-free apoA-I, when incubated with fibroblasts, will produce lipoproteins of prea mobility (Asztalos, B. F., et al. 1997. Arterioscler. Thromb. Vasc. Biol. 17: 1630–1636). In order to understand the nature of these prea particles, we further characterized their lipid content. The prea particles are high density lipoproteins, having a median density of 1.08 g/m...

consequences that lead to an increase in risk of atherosclerosis patients with epilepsy, several studies have reported the commonly used antiepileptic drugs like phenytoin, and carbamazepine serum total Cholesterol, High Density Lipoproteins Cholesterol (HDL-C) Low lipoprotein ( LDL-C ) levels Triglyceride (TG) .The aim this study assess compare lipid profile young adult treated by anti-epilept...