The binding interaction of novel podophyllotoxin derivative, (3R,4R)-4-((benzo[d][1,3]dioxol-5-yl)methyl)-dihydro-3-(hydroxy(3,4-dimethoxyphenyl) methyl) furan-2(3H)-one (PPT), with calf thymus DNA (ctDNA) has been examined using UV-Visible absorption spectrophotometry, fluorescence spectroscopy, viscosity measurement and molecular docking studies. UV-Vis absorption results showed hyperchromic ...

Threading intercalators are a novel class of materials that carry two substituents along the diagonal positions an aromatic ring. When bound to DNA, these project out in DNA grooves. Tetranuclear complexes appear be promising threading for developing therapeutics against cancer and viral infections require high nucleic acid binding affinity. The objective this work was prepare thiosemicarbazone...

In this paper, three complexes of type [Co(en)(2)PIP](3+)(PIP=2-phenylimidazo[4,5-f][1,10,] phenanthroline)(1), [Co(en)(2)IP](3+) (IP = imidazo[4,5-f][1,10,] phenanthroline)(2), and [Co(en)(2)phen-dione](3+)(1,10 phenanthroline 5,6,dione)(3) have been synthesized and characterized by UV/VIS, IR, (1)H NMR spectral methods. Absorption spectroscopy, emission spectroscopy, viscosity measurements, a...

Calf thymus chromatin was partially deproteinized by 0.6-1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitro RNA synthesis in an amount proportional to the fraction of exposed D...

DMBA, 7,12-dimethylbenz[a]anthracene, is a widely studied polycyclic aromatic hydrocarbon that has long been recognized as a probable human carcinogen. It has been found that DMBA is phototoxic in bacteria as well as in animal or human cells and photomutagenic in Salmonella typhimurium strain TA102. This article tempts to explain the photochemistry and photomutagenicity mechanism. Light irradia...

The two arginine-rich histones III and IV, isolated from pea buds and calf thymus glands, were separated by preparative disc electrophoresis and each was shown to be homogeneous by amino-terminal and carboxyl-terminal analysis. Homologous arginine-rich components from calf thymus and pea bud were found to be extremely similar or identical in their aminoand carboxyl-terminal amino acids, amino a...

CC-1065 is a potent antitumor antibiotic which is cytotoxic to P388 and L1210 leukemia cells in vitro and in vivo. CC-1065 covalently binds to calf thymus DNA preferentially to adenine-thymine regions at N3 of adenine. Here, we compare CC-1065 interaction with P388-derived chromatin, DNA, and histones as measured by electronic absorption and circular dichroism. Two CC-1065 analogues (U-71,184 a...

Background: Development of safe radioprotector is a challenging task. In this study radioprotective effect of sucrose has been demonstrated in calf thymus DNA (CtDNA). Sucrose is a free radical scavenger and also acts as osmolyte and therefore can influence the water activity around DNA and effects of radiation on DNA. Hoechst 33258 was used to probe the possible alteration in physicochemical p...

A stable, water-soluble substance which possesses potent antimycobacterial activity under certain conditions in vitro has been prepared from calf thymus. This substance has been tentatively named thymus peptide. In final concentrations of 1 to 10 microg. per ml. of an albumin medium it inhibits the growth of various strains of mammalian mycobacteria, but manifests only little or no inhibitory a...

Histone fraction F2A1 has been isolated and purified from macronuclei of the ciliate Tetrahymena pyriformis. It migrates as a single species on sodium dodecyl sulphate-acrylamide gel electrophoresis, with a molecular weight indistinguishable from that of calf thymus F2A1. The solubility properties of Tetrahymena F2A1 are also similar to those of calf thymus F2A1. Electrophoretic analyses on ure...