Different experimental conditions have been compared to achieve a high efficiency in embryogenic calli initiation from 'Macabeo' and 'Tempranillo' anthers. Specifically, two stages of anther development were tested (corresponding to tetrad cells or uninucleate pollen), and direct culture of anthers was compared to culture after a cold treatment of inflorescences (4 °C during 48 h). In addition,...

Tobacco mosaic virus infection of Nicotiana tabacum mesophyll protoplasts did not affect the pattern of chloroplast or total cellular DNA synthesis for at least 120 h when compared with that of mock-infected cells. Calli derived from infected protoplasts often showed large amounts of tobacco mosaic virus RNA and coat protein.

Blackberries are fruits produced worldwide, with 25 % of their production centered in Mexico, Central and South America. Tropical highland blackberry is a fruit that can potentially enhance human health, due to their high content in phenolic compounds, which include anthocyanins, phenolic acids, tannins (gallotannins and elagitannins) and flavonoids. Therefore, the overall aim of this study is ...

In this study, gamma rays were used to irradiate embryogenic calli induced from cotyledon explants of Panax ginseng Meyer. After the embryogenic calli were irradiated, they were transferred to adventitious roots using an induction medium; next, mutated adventitious root (MAR) lines with a high frequency of adventitious root formations were selected. Two MAR lines (MAR 5-2 and MAR 5-9) from the ...

The present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of Fritillaria imperialis L. There is no published method recommended for protoplast isolation and regeneration from Fritillaria imperialis L. A range of factors, which influence the success of isolation and regeneration of F. imperialis protoplasts, were investigated. From the results obta...

BACKGROUND Obtaining dedifferentiated cells (callus) that can regenerate into whole plants is not always feasible for many plant species. Sugar beet is known to be recalcitrant for dedifferentiation and plant regeneration. These difficulties were major obstacles for obtaining transgenic sugar beets through an Agrobacterium-mediated transformation procedure. The sugar beet line 'NK-219mm-O' is a...

Drought and salinity can be serious problems for agricultural productivity in certain planet areas. Leptosphaeria maculans is the causative agent of blackleg crucifer plants. In this work, a novel methodology studying effects fungal metabolites (volatile non-volatile) on plant calli presence abiotic stresses presented, by using L. maculans, tomato calli, drought stresses. way, study has reporte...

thyme (thymus vulgaris l.) is an aromatic and medicinal plant which is very important for the herbal industry. in this research, in vitro selection for nacl tolerance was investigated in this plant. hypocotyl and shoot explants (apical meristem and cotyledonary leaves) of sterilized seedlings were cultured in ms medium supplemented with different combinations of 2, 4-dichlorophenoxyacetic acid ...

Callus cultures derived from pith tissue of Nicotiana tabacum were grown on two media either under continuous illumination or in complete darkness. The first medium limited greening ability of callus grown in the light (3 milligrams per liter naphthalene acetic acid, 0.3 milligram per liter 2-isopentenylaminopurine, Murashige and Skoog salts, and 2% sucrose). The second medium encouraged chloro...