Group B streptococcus (GBS) capsular serotypes are major determinants of virulence and affect potential vaccine coverage. Here we report a whole-genome-sequencing-based method for GBS serotype assignment. This method shows strong agreement (kappa of 0.92) with conventional methods and increased serotype assignment (100%) to all 10 capsular types.

conclusions the outcome of this study indicates a higher discriminatory power of the rflp analysis based on the spa gene compared to the coa gene. moreover, the results of our study reveal that the resistance rate of s. aureus to some antimicrobial agents including linezolid is a growing concern. background antibiotic resistant staphylococcus aureus is a serious public health problem worldwide....

Colistin is one of the last-resort therapeutic antibiotics used to control carbapenem-resistant Klebsiella pneumoniae. However, emergence colistin-resistant strains with high mortality and morbidity has become a new challenge. Therefore, it crucial characterize genotypic features carbapenem- K. pneumoniae (CCKP) isolates implement public health intervention strategies. In this study, we analyze...

Abstract In this study, we focused on the emergence of extensively drug-resistant (XDR), pandrug-resistant (PDR), and hypervirulent Klebsiella pneumoniae (hvKP) in Iran. During 2018 to 2020 a total 52 K. isolates were collected from different clinical specimens. The hvKP identified by PCR amplification virulence capsular serotype-specific genes. Hypermucoviscous (hmKP) string test. Carbapenem-r...

BACKGROUND Group B Streptococcus (GBS) (Streptococcus agalactiae) is an important pathogen in neonates, pregnant women, and adults with underlying disease. METHODS Fifty clinical isolates were collected during the period 2001-2004 and analyzed by multilocus sequence typing and capsular serotyping. RESULTS The six major sequence types (STs) identified by multilocus sequence typing were ST1, ...

The vast majority of streptococci colonizing the human upper respiratory tract are commensals, only sporadically implicated in disease. Of these, the most pathogenic is Mitis group member, Streptococcus pneumoniae Phenotypic and genetic similarities between streptococci can cause difficulties in species identification. Using ribosomal S2-gene sequences extracted from whole-genome sequences publ...

A national study was undertaken to investigate the incidence of invasive pneumococcal disease in the Republic of Ireland and to examine the associated isolates. In 1999, 144 S. pneumoniae isolates, all recovered from cases of invasive disease, were received from 12 microbiology laboratories. The incidence of invasive pneumococcal disease was estimated to be 6.6/100000 population. All isolates w...

Streptococcus agalactiae, commonly known as group B streptococcus (GBS), is a cause of infectious disease in numerous animal species. This study examined the genetic relatedness of piscine, dolphin and human GBS isolates and bovine GBS reference strains from different geographical regions using serological and molecular serotyping and multilocus sequence typing (MLST) techniques. Piscine isolat...

As early as 1902, Neufeld observed that the capsulated pneumococcus showed a peculiar change when it was treated with its specific antiserum, but for a number of years the important practical value of this reaction had not been realized and until our knowledge that there are several serological types of pneumococcus was fully established this reaction had not been utilized in the typing of the ...

streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. capsular polysaccharide (cps) of s. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of s. iniae infection in aquatic animals. the cpsdgene was determined to be highly conservative in capsule polysaccharide operon. prokaryotic expression of th...