The neurotrophin receptor p75(NTR) conveys multiple signals via its intracellular death domain. However, how the death domain is activated and interacts with downstream adaptors remains unclear. Here, we report two crystal structures of the p75(NTR) death domain in the form of a non-covalent asymmetric dimer and a Cys379-Cys379 disulfide bond linked symmetric dimer, respectively. These two dime...

Onconase((R)) (ONC) is a homolog of ribonuclease A (RNase A) that has unusually high conformational stability and is toxic to human cancer cells in vitro and in vivo. ONC and its amphibian homologs have a C-terminal disulfide bond, which is absent in RNase A. Replacing this cystine with a pair of alanine residues greatly decreases the conformational stability of ONC. In addition, the C87A/C104A...

Disulfide bonds are widespread in peptides and proteins where they strengthen conformation and lead to increased rigidity and proteolytic stability. The number of disulfide-rich therapeutics currently available is increasing, such as the FDA-approved peptide drugs Prialt (ziconotide) and Linzess (linaclotide), both of which contain 3 disulfide bonds. In order to prepare disulfide-rich peptides,...

2-Amino-4-phenyl-5,6,7,8-tetrahydroquinoline-3-carbonitrile (3) was synthesized by treating cyclohexanone (1) with 2-benzylidenemalononitrile (2) in the presence of ammonium acetate. The reactivity of compound 3 towards dimethylformamide dimethyl acetal (DMF-DMA), carbon disulfide, urea, thiourea, formamide, formic acid, acetyl chloride and isothiocyanate were studied. In addition, the antimicr...

This report summarizes work, done at Philco Applied Research Laboratory under Contract Nonr-4850(00). Extensive measurements were taken on the conversion efficiency and threshold of stimulated Raman emission in benzene, nitrobenzene, toluene, and carbon disulfide. It has been shown that the self-focusing action of a laser beam is responsible for the onset of stimulated Raman emission.