The Saccharomyces cerevisiae myosin-V, Myo2p, is essential for polarized growth, most likely through transport of secretory vesicles to the developing bud. Myo2p is also required for vacuole movement, a process not essential for growth. The globular region of the myosin-V COOH-terminal tail domain is proposed to bind cargo. Through random mutagenesis of this globular tail, we isolated six new s...

In mammalian cells, endocytosed cargo that is internalized through clathrin-coated pits/vesicles passes through early endosomes and then to late endosomes, before delivery to lysosomes for degradation by proteases. Late endosomes are MVBs (multivesicular bodies) with ubiquitinated membrane proteins destined for lysosomal degradation being sorted into their luminal vesicles by the ESCRT (endosom...

Newly synthesized proteins are sorted into COPII-coated transport carriers at the endoplasmic reticulum (ER). Assembly of the COPII coat complex, which occurs at ER exit sites (ERES), is initiated by membrane association and GTP loading of SAR1, followed by the recruitment of the SEC23-SEC24 and SEC13-SEC31 subcomplexes. Both of these two subcomplexes stimulate GTP hydrolysis and coat disassemb...

The import and export of goods normally require the involvement of many different sectors from purchasing, manufacturing, transporting, inventory, distribution, etc. In order to have proper plans, accurately forecast the volume of imported-exported goods is the core issue. By comparing the performance of autoregressive integrated moving average (ARIMA) model, Grey model, and their joint Fourier...

The Ports of Long Beach and Los Angeles are the largest container port complex in North America and fifth largest in the world. Growth in U.S. Pacific Rim trade has put pressure on the landside transportation systems serving these two ports. In order to address this growth and mitigate environmental impacts, the ports are implementing new operational strategies and infrastructure improvements. ...

1987a). Sequence analysis of the heavy chain and com-Boston, Massachusetts 02115-5701 parisons with sequences of other proteins have not re-† Howard Hughes Medical Institute and vealed internal repeats, nor have they shown recogniz-‡ Children's Hospital able motifs or domains (Kirchhausen et al., 1987a). Laboratory of Molecular Medicine Rapid assembly and disassembly of coats are central Boston...

1987a). Sequence analysis of the heavy chain and com-Boston, Massachusetts 02115-5701 parisons with sequences of other proteins have not re-† Howard Hughes Medical Institute and vealed internal repeats, nor have they shown recogniz-‡ Children's Hospital able motifs or domains (Kirchhausen et al., 1987a). Laboratory of Molecular Medicine Rapid assembly and disassembly of coats are central Boston...

In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs). To date, the molecular assembly of such mRNPs is not well understood and most details on cargo specificity remain unresolved. We used ASH1-mRNA transport in ye...