CRISPR-Cas systems are one of the most widespread phage resistance mechanisms in prokaryotes. Our lab recently identified the first examples of phage-borne anti-CRISPR genes that encode protein inhibitors of the type I-F CRISPR-Cas system of Pseudomonas aeruginosa. A key question arising from this work was whether there are other types of anti-CRISPR genes. In the current work, we address this ...

Bacteria and Archaea encode clustered, regularly interspaced, short palindromic repeat (CRISPR) systems to confer adaptive immunity to invasive viruses and plasmids. Recent studies of CRISPR systems revealed that diverse CRISPR-associated (Cas) interference modules often coexist in different organisms but functions of cas genes have not been dissected in any of these systems. The crenarchaeon S...

in this paper‎, ‎based on cas wavelets we present quadrature rules for numerical solution‎ ‎of double and triple integrals with variable limits of integration‎. ‎to construct new method‎, ‎first‎, ‎we approximate the unknown function by cas wavelets‎. ‎then by using suitable collocation points‎, ‎we obtain the cas wavelet coefficients that these coefficients are applied in approximating the unk...

The human bacterial pathogen Listeria monocytogenes is emerging as a model organism to study RNA-mediated regulation in pathogenic bacteria. A class of non-coding RNAs called CRISPRs (clustered regularly interspaced short palindromic repeats) has been described to confer bacterial resistance against invading bacteriophages and conjugative plasmids. CRISPR function relies on the activity of CRIS...

Carbonic anhydrases (CAs) play fundamental roles in several physiological events, and emerging evidence points at their involvement in an array of disorders, including cancer. The expression of CAs in the different cells of teeth is unknown, let alone their expression patterns during odontogenesis. As a first step towards understanding the role of CAs during odontogenesis, we used immunohistoch...

Abstract The widespread and versatile prokaryotic CRISPR–Cas systems (clustered regularly interspaced short palindromic repeats associated Cas proteins) constitute powerful weapons against foreign nucleic acids. Recently, the single-effector nuclease Cas12a that belongs to type V system was added enzymes repertoire employed for gene editing purposes. is a bilobal enzyme composed of REC Nuc lobe...

Syndrome de la queue de cheval. A propos de 4 cas.  Les auteurs rapportent quatre observations cliniques concernat des malades dont les pre­miers symptomes se manifesterent par des al­gies longitemps considerees comme "sciatique·s".  L' etude neurologique de ces cas demontrait qu'en realite "les algies sciatiques" n'etaient que des signes mono ou pluri-radiculaires en rapport avec un syndrome...

the objective of this research is to identify the characteristics of cost accounting systems (cas) in manufacturing companies located in the state of fars. the research is designed to answer the following questions: 1- what kind of management information systems (mis) are in - use. 2-what information are provided by these systems. 3-how - offen financial information. are issues. 4- what groups ...

Cell migration and wound contraction requires assembly of actin into a functional myosin motor unit capable of generating force. However, cell migration also involves formation of actin-containing membrane ruffles. Evidence is provided that actin-myosin assembly and membrane ruffling are regulated by distinct signaling pathways in the migratory cell. Interaction of cells with extracellular matr...