Use of alternative donors/sources of hematopoietic stem cells (HSC), such as cord blood (CB) or HLA-haploidentical (Haplo)-related donors, is associated with a significant delay in immune reconstitution after transplantation. Long-term T-cell immune reconstitution largely relies on the generation of new T cells in the recipient thymus, which can be evaluated through signal joint (sj) and beta T...

Expression of the bacterial insertion sequence IS911 transposase in vivo leads to excision and circularization of IS911-based transposons. We show here that transposase produces an unusual molecular form generated by single-strand cleavage, transfer, and ligation of one end of the element to the opposite end. When the transposon is carried by a circular plasmid, this results in the formation of...

Abstract Background Patients with COVID-19 (COVID) may develop acute respiratory distress syndrome or without sepsis, coagulopathy and visceral damage. While chest CT scans are routinely performed in the initial assessment of patients severe pulmonary forms, thymus involvement reactivation have not been investigated so far. Methods In this observational study, we systematically scored enlargeme...

The effect of steroid treatment on the thymic output of T cells was examined in an avian model. Recent thymic emigrants in chickens transiently express the chicken T cell Ag 1 thymocyte marker, and thymic function can be monitored indirectly by measuring the levels of TCR gene rearrangement excision circles in peripheral T cells. Both parameters were used to show that intensive steroid treatmen...

The maize Ac/Ds transposable elements are thought to transpose via a cut-and-paste mechanism, but the intermediates formed during transposition are still unknown. In this work we present evidence that circular Ac molecules are formed in plants containing actively transposing elements. In these circles, transposon ends are joined head-to-head. The sequence at the ends' junction is variable, cont...

TCRb, d and c chain genes are assembled and expressed in double-negative thymocytes prior to ab or cd T cell lineage commitment. Thus, cells committed to the ab T cell lineage can possess completely assembled TCRd and/or TCRc chain genes. However, these genes are not expressed. TCRc chain gene expression may be silenced through the activity of a cis-acting silencer element. In the TCRa/d locus,...

Newborn screening (NBS) programs are an anomaly in medicine—a universally applied, state-mandated evaluation of healthy individuals. The program had its origin in the presymptomatic diagnosis of phenylketonuria (1 ), but as technologies for the use of dried blood spots (DBS) have improved, there has been a rapid expansion of the number of disorders evaluated, as well as an increase in the gover...

The aim of this study was to evaluate thymus function in mice with hypertension. A total of 60 C57BL/6J mice were randomized into control, sham surgery and two-kidney, one-clip groups (n=20 in each). At 4 or 8 weeks after surgery, mice were sacrificed, and blood, spleens, kidneys and thymuses were harvested. The results of reverse transcription-quantitative polymerase chain reaction analysis re...

Purpose Considering the clear emerging role of the DNA repair and the cell cycle checkpoints as predictive, prognostic and therapeutic targets in cancer there is a need to better characterized human tumours to define sub-sets of patients that would benefit of a particular treatment modality. The aim of the present work is to characterize molecularly a cohort of Triple Negative Breast Cancers (T...