Overexpression of several aquaporins (AQPs) has been reported in different types of human cancer but their role in carcinogenesis, for example in the cervix, have yet to be clearly defined. In this study, expression of AQPs in cervical carcinomawas investigated by real-time PCR, immunofluorescent and immunohistochemical assays and evaluated for correlations with clinicopathologic variables. AQP...

PURPOSE In clinical practice, radiation therapy often fails in cervical carcinoma stage IIIB and there is a need to develop a predictive assay for prognosis of radiation treatment outcome in cancer patient. We have attempted to evaluate the relevance of changes in Membrane Fluidity (MF) and associated apoptotic cell death in cervical cancer cells after first fractionated dose of radiation thera...

Interleukin 6 (IL-6) is a multifunctional cytokine which has recently been shown to act in vitro as a growth factor for cervical carcinoma cell lines. This prompted us to measure IL-6 gene expression using a new quantitative polymerase chain reaction assay in 13 invasive cervical cancers, 5 cases of cervical intraepithelial neoplasia, and 2 normal cervix. A significant increase in the expressio...

To establish Lactate dehydrogenase (LDH) isoenzyme is a biochemical tumor marker in cervical carcinoma patients for diagnosis and treatment monitoring of the disease. The Serum and cervical tissue LDH isoenzyme was analyzed qualitatively by Poly Acrylamide Disc Gel Electrophoresis method. The total LDH activity was estimated quantitatively by the method of UV spectrophotometry. 50 untreated cer...

The human papillomavirus (HPV)16 E6 and E7 correlation with chemokine ligand (CCL)20 expression and Langerhans cells (LCs) in cervical lesions was investigated. We enrolled 43 patients with surgically treated cervical lesions from the Department of Gynecology in our hospital, and 20 controls without cervical lesions. Subjects were divided by pathology: HPV16(-) and HPV16(+) normal cervical grou...

abstract ureteralstump’s metastasis of renal cell carcinoma is rare. we report a32-year-old femalewith a huge metastatic involvement of the ureteralstump and utero-cervical structures about 2 years after a left radical nephrectomy. she underwent en-bloc resection of the massin conjunctionwith total ureterectomy, bladder cuff excision and hysterectomy.the patient was referred to oncology departm...

OBJECTIVE The abnormal expression of fragile histidine triad (FHIT) gene has been frequently reported in a variety of epithelial malignancies including cervical carcinoma. Furthermore, in a recent study it was proposed that transcriptional inactivation of FHIT, as a consequence of aberrant 5'-CpG island methylation, plays an important role in the carcinogenesis of human cervical carcinoma. The ...

OBJECTIVE The aim of this study was to determine whether the activity of topoisomerase I (topo I), the target of the anti-neoplastic drug camptothecin (CPT), is elevated in cervical cancer and whether CPT can radiosensitize cervical tumors. METHODS The topo I activity of 11 normal cervix and 30 cervical carcinoma tumors was assayed by measuring the relaxation of supercoiled DNA. Subconfluent ...

PURPOSE Acquisition of epithelial-mesenchymal transition (EMT) by primary carcinoma cells is associated with disrupted epithelial integrity, local invasion, and ultimately metastasis. Little is known about the existence and function of EMT in cervical cancer. This study aims to investigate the regulation of EMT in cervical squamous cell carcinoma. EXPERIMENTAL DESIGN We investigated the molec...

Objectives: Presence/absence of cervical lymph node metastasis plays a critical role in prognosis and survival of patients with oral squamous cell carcinoma (SCC). This study was designed to assess the diagnostic accuracy of computed tomography (CT) scan for detection of cervical lymph node metastasis in oral SCC in comparison with histopathological analysis after neck dissection Methods: In...