background bacterial resistance to antibiotics has become a major source of concern for public health. pseudomonas aeruginosa strains are important opportunistic pathogens. these bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics. objectives the present study was performed to evaluate the frequency of kpc in p. aeruginosa isolated from clinical samples of...

objectives the objective of this study was to develop the primers for single and/or multiplex pcr amplification assays for simultaneous identification of class a, class b, and class d carbapenem hydrolyzing β-lactamases in enterobacteriaceae and then to evaluate their efficiency. conclusions we have reported here a reliable method, composed of single and multiplex pcr assays, for screening all ...

conclusions the determination of serotype and surface proteins of gbs strains distribution would ‎be ‎relevant ‎for the future possible formulation of a gbs vaccine. patients and methods in this cross-sectional study, a total of 100 gbs strains isolated from 237 pregnant and 413 non-pregnant women were investigated for molecular capsular serotypes and surface protein genes using the multiplex p...

conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

background the occult hepatitis b infection (obi) is defined as the presence of hepatitis b virus (hbv) dna in the sera or in the liver biopsy and the absence of hepatitis b surface antigen (hbsag) by serological test. objectives the current study aimed to evaluate the occult hbv infection by polymerase chain reaction (pcr) and determine hbv genotyping among the patients with abnormal alanine t...

conclusions according to this research and prevalence of hhv-6 in children, evaluation of csf (detecting the hhv-6 dna by pcr) is recommended in patients younger than 13 months with possible encephalitis. results from a total of 114 patients, hhv-6 was detected in 10 (8.8%), 90% of which were boys with mean age 7.7 months and median of 7.5 months. no significant differences were found in clinic...