A factor which specifically stimulates the growth of sensory and sympathetic nerve cells has been under investigation in this laboratory for a number of years. The growth stimulation is demonstrable both in tissue culture, on isolated nerve ganglia, and in the living chick embryo. The factor was originally discovered in certain mouse sarcomas (2-5) and the biological activity was found to resid...

701 T HE CHICK EMBRYO 15 used widely in studying drugs for teratogenic activity. Chemicals, which have been injected at the same period of incubation of the chick eml)ryo, may produce separate and characteristic patterns of developmental abnormalities. The effects of many drugs, such as insulin, azasenine, sulfanilamide, 4-aminopteroyl glutamic acid, 8-azaguanine, physostigmine, thallium, lead,...

biologic crystals and particles (BCP) in this report were shown in the first paper of this series (13) as large (up to 300 z in length) RNA-staining objects (pyronin response; controlled by RNase) which appeared in cul tures of chick embryo tissues viewed by phase-contrast microscopy. Their pleomorphic shapes were described as either semirigid tubules, right-handed helices (12), ribbons, rhombo...

Chick embryo cells transformed by either of two strains of Rous sarcoma virus (Bryan high titer or Schmidt-Ruppin) have low levels of alkaline phosphatase activity compared with nontransformed chick embryo cells. Essentially no differences in acid phosphatase activity were observed between these transformed and nontransformed cells. A virus mutant, RSV-BH-Ta, induces temperature-dependent trans...

  Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study.   Materials and Methods: White Leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in D...

We have used fluorescein isosthiocyanate-conjugated gelatin (FITC-gelatin) (1 mg/ml) to localize cell surface fibronectin in unfixed live cells in cultures. FITC-gelatin stains the fibronectin matrix on primary cultures of rat and chick embryo fibroblasts as well as untransformed, established cell lines. In live cultured cells, fibronectin in many areas of the extracellular matrix is inaccessib...

BACKGROUND The biological mechanisms by which environmental metals are associated with birth defects are largely unknown. Systems biology-based approaches may help to identify key pathways that mediate metal-induced birth defects as well as potential targets for prevention. OBJECTIVES First, we applied a novel computational approach to identify a prioritized biological pathway that associates...

Developmental biology research has used various avian species as model organisms for studying morphogenesis, with the chick embryo being used by the majority of groups. The focus on the chick embryo led Hamburger and Hamilton to develop their definitive staging series nearly 60 years ago and this series is still the mainstay of all laboratories working with avian embryos. The focus on the chick...

introduction: depending on mode, intensity and length of application, and stage of development, the low-frequency electromagnetic fields (lfes) can induce a wide variety of biological effects in organisms. the chick limb bud is a powerful experimental system for study pattern formation in vertebrate embryo. the aim of this study was to examine the effects of an application lfes (50hz, 50g) on c...