A toxin that causes morphological changes in Chinese hamster ovary cells was isolated from a culture filtrate of Shigella dysenteriae 1. It was partially purified by successive column chromatography on diethylaminoethyl-cellulose, Bio-Gel A-5m, and hydroxylapatite. The toxin was separated from neurotoxin by diethylaminoethyl-cellulose column chromatography and was not lethal for mice.

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

introduction: neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. also, neurotropic factors are growth factors like neurotrophins that help neurons survive. moreover, neurotrophins differentiate between progenitor cells so that neurons are formed. despite the fact that the majority of mammalian brain neurons are produced p...

Screening of different yeast species showed that they are able to synthesize hydroxymethylglutaryl-CoA (HMGCoA) reductase inhibitors. Crude methanol extracts and the purified inhibitors from Pichia labacensis and Candida cariosilignicola were tested for their biological activity on the solubilized microsomal HMGCoA reductase from Chinese hamster ovary cells. Identification of the inhibitors was...

We have created a genome-scale network reconstruction of chinese hamster ovary (CHO) cell metabolism. Existing reconstructions were improved in terms of annotation standards, to facilitate their subsequent use in dynamic modelling. The resultant network is available from ChoNet (http://cho.sf.net/).

Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation...

A series of observations have indicated that tau, one of the major microtubule-associated proteins, is involved in neuronal cell morphogenesis and axonal maintenance. Tau is also the major component of paired helical filaments found in brains affected by Alzheimer's disease. To explore an as yet unidentified role of tau in vivo, approximately 11,000 mRNAs were profiled from tau-deficient mouse ...

We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the initial attachment and subsequent spreading of these cells on substrata composed of intact fibrone...

Mammalian cell cultures are the preferred expression systems for the production of biopharmaceuticals requiring posttranslational processing. Usually, cell cultures are cultivated in medium supplemented with serum, which supports cell proliferation, viability, and productivity. However, due to scientific and regulatory concerns, serum-free conditions are required in recombinant protein producti...

Citation for published version (APA): Shimozawa, N., Suzuki, Y., Zhang, Z., Imamura, A., Kondo, N., Kinoshita, N., ... Wanders, R. J. A. (1998). Genetic basis of peroxisomes-assembly mutants of humans, Chinese hamster ovary cells, and yeast: identification of a new complementation group of peroxisome-biogenesis disorders apparently lacking peroxisomal-membrane ghosts (letter). American Journal ...