Despite the growing interest to explore untapped microbial gene and protein diversity, no single platform has been able to acquire both gene and protein information from just a few cells. We present a microfluidic system that simultaneously performs on-chip capillary electrophoresis for protein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same coh...

Fluorescence detection is known to be one of the most sensitive among the different optical sensing techniques. This work focuses on excitation and detection of fluorescence emitted by DNA strands labeled with fluorescent dye molecules that can be excited at a specific wavelength. Excitation occurs via optical channel waveguides written with femtosecond laser pulses applied coplanar with a micr...

due to high resolution power of sulfated cyclodextrins (hs-cds), utilization of these selectors for chiral resolution of 7 basic and 2 zwitterionic drugs have been examined. experiments were performed on a hp3dce instrument equipped with an on-column diode array uv absorbance detector. fused silica capillaries with an inner diameter of 50 ?m, an outer diameter of 365 ?m, and a total length of 4...

Since the concept of miniaturized total analysis systems (microTAS) was invented, a great number of microfluidic devices have been demonstrated for a variety of applications. However, an important hurdle that still needs to be cleared is the connection of a microfluidic device with the rest of the world, which is often referred to as the macro-to-micro interface, interconnect, or world-to-chip ...

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Here we show that a closed pneumatic microvalve on a PDMS chip can serve as a semipermeable membrane under an applied potential, enabling current to pass through while blocking the passage of charged analytes. Enrichment of both anionic and cationic species has been demonstrated, and concentration factors of ~70 have been achieved in just 8 s. Once analytes are concentrated, the valve is briefl...

A capacitively coupled contactless conductivity detector produced in Low Temperature Co-fired Ceramic (LTCC) technology for microchip capillary electrophoresis (CE) is reported in this work. Electrodes located outside the measurement channel are used to sense impedance variations caused by conductive ions. The application of LTCC in combination with contactless conductivity detection is very pr...

Advances in many areas of genomics research are heavily rooted in engineering technology, from the capillary electrophoresis units used in large-scale DNA sequencing projects, to the photolithography and robotics technology used in chip manufacture, to the confocal imaging systems used to read those chips, to the beam and detector technology driving high-throughput mass spectroscopy. Further ad...