Chitinases are the hydrolytic enzymes which protect plants against pathogen attack. However, the precise role of chitinases in disease resistance has not been explored in wheat. In the present study, in silico approach, including secondary structure analysis, detailed signature pattern study, cis-acting regulatory elements survey, evolutionary trends and three-dimensional molecular modeling was...

A new chitinase isozyme (Chitinase A), which had only one optimum pH toward a long substrate, glycolchitin, was purified from the peel of yam tuber by CTAB (hexadecyl trimethyl ammonium bromide) treatment and ammonium sulfate fractionation, followed by column chromatography on DEAE-Cellulofine A-500, chromatofocusing, and gel filtration on Sephacryl S-100. The molecular weight was 28,000 by SDS...

Chitinases (EC 3.2.1.14) are hydrolytic enzymes found in different organisms. In plants, they have been described in different tissues and organs, including seeds. This study was triggered by the isolation of a 30-kDa thermostable chitinase from Adenanthera pavonina L. seeds. The enzyme was submitted to N-terminal amino acid sequencing, and the analysis revealed a high degree of homology with c...

Fish express two different chitinases, acidic fish chitinase-1 (AFCase-1) and acidic fish chitinase-2 (AFCase-2), in the stomach. AFCase-1 and AFCase-2 have different degradation patterns, as fish efficiently degrade chitin ingested as food. For a comparison with the enzymatic properties and the primary structures of chitinase isozymes obtained previously from the stomach of demersal fish, in t...

Based on first principles and molecular mechanics calculations, we conclude that the mechanism of hevamine (a family 18 chitinase) involves an oxazoline ion intermediate stabilized by the neighboring C2' acetamido group. In this intermediate, the acetamido carbonyl oxygen atom forms a covalent bond to C1' of N-acetyl-glucosamine and has a transferred positive charge from the pyranose ring onto ...

Chitin, the most abundant bio-polymer in seawater, may be utilized by various microorganisms as a carbon source. Vibrios have been regarded one of main groups chitin consumers marine cycle and chitinase producers. The organisms are widely distributed aquatic environment. However, co-working mechanism between their chitinases, whether chitinase’s diversity contributes to adaption environment, ne...

Abstract Background This study was carried out to investigate the antifungal activity of chitinase produced by Streptomyces species isolated from grassland soils against selected pathogenic fungi, namely: Magnaporthe oryzae , Fusarium graminearum Rhizoctonia solani Puccinia and Botyrtis cinerea . Chitinase albus purified consecutively with dialysis, gel filtration ion- exchange chromatography, ...

Shrimp shell waste are potential to be processed further into value-added products, such as N-acetylglucosamine (GlcNAc). In the production of GlcNAc, biological approach is preferred and environmentally friendly chemical treatment. Chitinase an enzyme that plays a vital role in bioprocessing shrimp GlcNAc. Previously, Bacillus cereus SMG 1.1 was isolated from fermented paste (terasi) showed ab...

Baculovirus chitinases and other family 18 glycohydrolases have been shown to possess both exo- and endochitinase activities when assayed against fluorescent chito-oligosaccharides. Homology modelling of the chitinase of Epiphyas postvittana nucleopolyhedrovirus (EppoNPV) against Serratia marcescens chitinase A indicated that the enzyme possesses an N-terminal polycystic kidney 1 (PKD1) domain ...

A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) larvae gut discovered in mushroom compost. strain selected the source and labeled as RBLG1. It identified using 16s rDNAsequencing has strong homology to Bacillus cereus. Chitinase produced RBLG1 purified ammonium sulfate precipitation, ion exchange chromatography, gel filtration. The chitinase shows...