A polymerase chain reaction (PCR) method was compared to standard methods (cultures for Neisseria gonorrhoeae and Chlamydia trachomatis and an enzyme-immunoassay for C. trachomatis) in diagnosis of gonococcal and chlamydial urethritis in 40 male patients with urethritis. Gonococcal urethritis was diagnosed by detection of a 206 bp DNA fragment amplified by PCR with N. gonorrhoeae-specific prime...

Despite significant advances in our understanding of the biology and antigenic structure of Chlamydia trachomatis, and the epidemiology and clinical spectrum of chlamydial disease, the magnitude of morbidity from human chlamydial infections remains an important public health concern. Control of chlamydial disease will likely depend on a multidisciplinary approach, including the development of i...

abstract       background and objective: chlamydia trachomatis is one of the most common causes of genital infection in men and women. genital chlamydial infections in women are clinically asymptomatic in 70-80% of the cases; therefore, the lack of timely diagnosis and treatment leads to complications such as infertility and ectopic pregnancy. the aim of this study was to evaluate the frequency...

Chlamydiae are obligate intracellular bacteria causing mucosal infections. The leading agent Chlamydia trachomatis causes three clinical features in eyes: trachoma, neonatal, and adult inclusion conjunctivitis. A rare chlamydial conjunctivitis form called Lymphogranuloma Venereum can be venereally transmitted. Seldomly psittaci pneumonia may cause follicular Trachoma, the most sight-threatening...

Due to the global prevalence of Chlamydiae, exploring studies of diverse antichlamydial compounds is important in the development of effective treatment strategies and global infectious disease management. Chlamydiaceae is the most widely known bacterial family of the Chlamydiae order. Among the species in the family Chlamydiaceae, Chlamydia trachomatis and Chlamydia pneumoniae cause common hum...

background: chlamydia trachomatis is considered as an important cause of preventable sexually transmitted diseases worldwide. it is known to be of an obligate intracellular nature and enters its target cells via an endocytic process. as major outer membrane protein (momp) is one of the main candidates for the attachment and entry of chlamydia to the host cells we have tried to label the epitope...

Chlamydia trachomatis is an obligate intracellular pathogen that replicates within a parasitophorous vacuole termed an inclusion. The chlamydial inclusion is isolated from the endocytic pathway but fusogenic with Golgi-derived exocytic vesicles containing sphingomyelin and cholesterol. Sphingolipids are incorporated into the chlamydial cell wall and are considered essential for chlamydial devel...

Chlamydia trachomatis is an obligate intracellular bacterium that alternates between two metabolically different developmental forms. We performed fluorescence lifetime imaging (FLIM) of the metabolic coenzymes, reduced nicotinamide adenine dinucleotides [NAD(P)H], by two-photon microscopy for separate analysis of host and pathogen metabolism during intracellular chlamydial infections. NAD(P)H ...

Background Chlamydia pecorum is a globally significant livestock pathogen causing pathology and production losses. The on-farm infection and serological dynamics and the relevance of existing diagnostic tools for diagnosing C. pecorum in livestock remains poorly characterized. In this study, we characterized the antigen and antibody dynamics of this pathogen in a longitudinal study of prime lam...

The aim of our study was to evaluate whether in women with chlamydial cervicitis urogenital mycoplasmas and group B streptococci (GBS) colonization is found more often than among women with non-chlamydial cervicitis. This study included 351 (mean age 31.7 +/- 6.82) not pregnant, menstruating, sexually active women. We confirmed a high frequency (49.3%) of C. trachomatis infection among women wi...