Incubation at 37#{176}Cfor 30 mm or the addition of CaCl2 (5 mM) generated platelet-clumping activity in citrated plasma and EDTA plasma previously acidified to pH 5.5 and in which heparin was added to give a concentration of 0.5 mg/mi. Similar treatment could not produce platelet-clumping activity in saline or denatured plasma that had been heated to 56#{176}C.Heparin, chondroitin sulfate B, o...

This study reports for the first time the complete liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and tandem mass spectrometry (MS/MS) analyses performed in negative ion mode of saturated unsulfated chondroitin oligosaccharides up to 16-mers and comparison with hyaluronic acid (HA) oligomers differing only in the nature of the hexosamine residue. MS/MS of the chondr...

Cultured human fetal lung fibroblasts produce some chondroitin sulfate proteoglycans that are extracted as an aggregate in chaotropic buffers containing 4 M guanidinium chloride. The aggregated proteoglycans are excluded from Sepharose CL4B and 2B, but become included, eluting with a Kav value of 0.53 from Sepharose CL4B, when Triton X-100 is included in the buffer. Conversely, some of the dete...

The use of sulfate of exogenous origin in the synthesis of chondroitin sulfate was suggested (1) and then demonstrated by the isolation of chondroitin sulfate-W from the cartilage of rats given sulfate-V6 (2, 3). Confirmatory reports have supplemented the earlier observations (4, 5). Autoradiography has been particularly effective in showing that administered sulfate-S36 is concentrated to a gr...

Glycosaminoglycans, mainly chondroitin 4-sulfate, are located in the primary granules of human myeloid cells. These polyanionic carbohydrates are believed to play an important role in leukocyte maturation and function. To study the effect of altered chondroitin sulfate metabolism on human promyelocytic leukemia cells, we have treated HL-60 cells with 4-methylumbelliferyl-$-D-xyloside. -DXylosid...

Microsomal membranes from chick embryo epiphyseal cartilage were fractionated by equilibrium sucrose-density-gradient centrifugation and assayed for GlcA (glucuronic acid) transferase I (the enzyme that transfers GlcA from UDP-GlcA to Gal-Gal-Xyl of proteochondroitin linkage region), for comparison with GlcA transferase II (the GlcA transferase of chondroitin polymerization). Gal(beta1-3)Galbet...

The synthesis of hyaluronic acid by cultured chondrocytes from the Swarm rat chondrosarcoma was examined using [3H]glucosamine as a precursor. [3H]Hyaluronate in samples was estimated as the specific unsaturated disaccharide released by incubation with chondroitinase ABC under conditions where all [3H]hyaluronate was converted to disaccharide even in the presence of excess chondroitin sulfate. ...

1. The effects of acid mucopolysaccharides and acid mucopolysaccharide-proteins on the size and rate of formation of fibril aggregates from collagen solutions in pH7.6 buffers were studied by turbidimetric and light-scattering methods. 2. Serum albumin, orosomucoid, methylated cellulose, chondroitin sulphate A and chondroitin sulphate C of molecular weight less than 20000, and hyaluronate of mo...

This paper deals with the possible relationship between a native, negatively charged polyelectrolyte, chondroitin sulfate, and sodium metabolism. The physical chemical property of decreased activity of the cations Na, K and Ca in chondroitin sulfate is described. The data of experiments indicating an alteration in the inueoprotein metabolism of the cartilage of the rabbit's ear in response to t...

This paper deals with the possible relationship between a native, negatively charged polyelectrolyte, chondroitin sulfate, and sodium metabolism. The physical chemical property of decreased activity of the cations Na, K and Ca in chondroitin sulfate is described. The data of experiments indicating an alteration in the inueoprotein metabolism of the cartilage of the rabbit's ear in response to t...