Biochemical variability among 20 Indian isolates of Sclerotinia sclerotiorum collected from different hosts/soil samples from different localities in India is reported. High Performance Liquid Chromatographic (HPLC) analysis of ethyl acetate fraction of culture filtrate, mycelia, sclerotia and sclerotial exudate showed 15~23 peaks but only 11 could be identified. They were tannic, gallic, oxali...

A procedure to identify dihydroflavonol (glucosides), cinnamic acids and anthocyanins in flowers of Petunia hybrida is presented. Nearly all known intermediates and anthocyanins ocurring in Petunia are separated on one column using different elution systems. Hydrolysis of extracts before HPLC and thin layer chromatography is in some cases necessary to make positive identification of flavonoids....

Ethanol and dichloromethane extracts of a Brazilian green propolis from Baccharis dracunculifolia were analyzed by HPLC-APCI-MS and GC-MS, respectively. The HPLCAPCI-MS technique, at the positive mode, furnished a complete and unequivocal chemical composition of the green propolis sample. It serves as fi ngerprint for different propolis samples. The composition of the ethanol extract consisted ...

In this study, the fatty acid compositions, lipid-soluble vitamins, sterols, flavonoids, and phenolic levels, as well DPPH radical scavenging activity of five different Dicranum species (D. majus, D. fuscencens, polysetum, scoparium tauricum) were examined. While acids evaluated by HPLC, compositions assessed gas chromatography. Based on study findings, it was determined that examined exhibit l...

Vegetables represent a major source of phenolic acids, powerful antioxidants characterized by an organic carboxylic acid function and which present multiple properties beneficial for human health. In consequence, developing new varieties with enhanced content in phenolic acids is an increasingly important breeding objective. Major phenolic acids present in vegetables are derivatives of cinnamic...

Chiral pyrrolidines and pyrrolizidines with spirooxindole ring systems are the central skeletons for numerous alkaloids and pharmacologically important compounds. Gelesmine, pseudotabersonine, formosanine, isoformosanine, morroniside and mitraphylline are some of the alkaloids containing spirooxindole ring systems. Derivatives of spirooxindole find very wide biological applications as anti micr...

Callus cultures were established in vitro from leaf explants of Malva neglecta and were sub-cultured several times until a rapid-growing cell line was obtained. The calluses were exposed to different doses of ultraviolet (UV) radiation as follows: 0, 432, 864, and 1296 J/m2 for UV B and 0, 612, 1284, and 1836 J/m2 for UV C, corresponding to 0, 30, 60, and 90 min radiations, in tandem. Exposure ...

Almendros, G., Martín, F. and González-Vila, F.J., 1987. Depolymerization and degradation of humic acids with sodium perborate. Geoderma, 39: 235-247. Two humic acids of different origin (peat and soil) were degraded with a 5 % sodium perborate solution (140 OC). This degradation process consists mainly of a stoichiometric production of hydrogen peroxide while the perborate is reacting with car...

This work aimed to evaluate the influence of root exudates from cover crops: Raphanus sativus, Avena strigosa and Vicia villosa on germination development Zea mays, Amaranthus spinosus Ipomoea grandifolia. The tests performed were gas chromatography with mass spectrometry, rate, vigor, protrusion dry matter. Aliphatic amino acids (Alanine, Glycine, Isoleucine, Leucine, Proline, Serine, Threonin...

The electrophilic nature of chalcones (1,3-diphenylprop-2-en-1-ones) and many other α,β-unsaturated carbonyl compounds is crucial for their biological activity, which is often based on thiol-mediated regulation processes. To better predict their biological activity a simple screening assay for the assessment of the second-order rate constants (k(2)) in thia-Michael additions was developed. Henc...