The objective of this study was to examine the effect of donor breed on pronuclear-stage embryo yield to be used for DNA microinjection in a transgenesis goat program. Twelve Canindé and twelve Saanen goats were heat synchronized using a progestagen-cloprostenol treatment. Forty-eight hours before the sponge removal, superovulation was induced with a total administration of 4.4 mg/kg bodyweight...

The objective was to evaluate the effect of the first artificial insemination (AI) on the number of viable and degenerated embryos, total embryos and unfertilized oocytes, and fertilization rate in Holstein donor cows. Cows (n=22) and heifers (n=50) were synchronized by the Crestar (Intervet International B.V., Boxmeer, Holland) protocol where 3 mg of norgestomet are implanted subcutaneously in...

This study aimed to assess the efficiency of deslorelin at inducing ovulation in crossbred cows previously exposed to intravaginal progesterone (P4). A total of 43 cows (Bos taurus × Bos indicus) were randomly divided into two groups, G1 (treated, n = 25) and G2 (control, n = 18). Both groups received an identical hormone protocol from day (D) 0 to D8: D0 = intravaginal P4 + estradiol benzoate ...

Luteolysis, which occurs in a cyclical way to remove luteal tissue, may be an example of physiological apoptosis which counterbalances rapid tissue growth after ovulation. Clusterin is a multifunctional glycoprotein expressed in different tissues undergoing apoptosis. In this study we investigated clusterin and LH receptor gene expression during luteolysis as potential regulators of tissue grow...

The objective of this study was to evaluate the efficiency of two doses of PGF associated or not to hCG on the associated reproductive parameters in dairy goats. A total of 29 goats received two doses of 30μg d-cloprostenol latero-vulvar at a 10 day intervals (Day 1 and Day 10). The does were allocated according to body weight and body condition score into two treatments, to receive hCG (250IU)...

Standard artificial insemination (AI) using a speculum in dairy goats does not result in acceptable fertility rates in nulliparous does. An explanation might be the difficulties to pass the cervical canal in nulliparous females with the insemination gun, increasing the time needed for semen deposition. Nulliparous Alpine dairy goats were used to evaluate whether time interval from insertion to ...