BACKGROUND One of the most challenging problems in tissue engineering is the establishment of vascular supply. A possible approach might be the engineering of microvasculature in vitro and the supply by engineered feeder vessels. METHODS An in vitro model for a small-diameter vessel was developed and made from adipose tissue stromal cells and human umbilical vein endothelial cells in a tube-l...

Bone's microporosity plays important roles in bone biology and bone mechanical quality. In this study, we explored the accuracy and reproducibility of nondestructive desktop μCT for 3D visualization and subsequent morphometric analysis of mouse cortical bone microporosity including the vascular canal network and osteocyte lacunae. The accuracy of measurements was evaluated in five murine fibula...

PURPOSE Photochemical cross-linking of corneal stromal collagen using riboflavin and ultraviolet irradiation is an evolving treatment for keratoconus. The purpose of the present study was to investigate the wound-healing process in rabbit corneas after cross-linking. METHODS Photochemical cross-linking was performed according to a standard protocol on the right eyes of eight male New Zealand ...

While biological evolution and learning are stepwise processes, classical Artiicial Neural Networks (ANNs) are designed and trained "in one go". The authors suggests that artiicial organisms whose neural architecture emerges via Darwinian evolution and which are trained "step by step" may be able to achieve richer functionality than is possible for conventional ANNs. The paper presents the Comp...

Image processing techniques are frequently used for extracting quantitative information (cell area, cell size, counting, etc.) from different types of microscopic images. analysis biology and tissue engineering is time consuming requires personal expertise. In addition, evaluation the results may be subjective. Therefore, computer-based learning applications have been rapidly developed in recen...

Conjunctival petechiae from 15 cases (cause of death: different natural and unnatural) were analysed using confocal laser scanning microscopy (CLSM) in order to visualize the kind of the damage within the vessel wall (diapedesis versus rhexis). The pathomorphological findings with multiple ruptures of vessels appearing to be filled to bursting point define the conjunctival petechiae as a rhexis...

A key role in the regulation of membrane traffic is played by the rab proteins, members of a family of ras-related small GTP-binding proteins. This family comprises at least 25 identified members, the intracellular localization of only a few of which has been investigated. rab6p has been shown to be distributed along the exocytic pathway in association with the medial and trans regions of the G...