Agents that alter the dynamics of hemostasis form an important part in management of conditions such as atherosclerosis, cerebrovascular disease, and bleeding diatheses. In this study, we explored the effects of heparin and tranexamic acid on the efficiency of blood coagulation. Using optical tweezers, we evaluated the pN-range micro-interaction between coagulating red blood cells (RBCs) by mea...

Since the first description of spontaneous mast cell tumors (mastocytomas) of dogs (2), subsequent studies have been concerned with the morphology and behavior of the neoplastic mast cells in tissue culture (11). Chemical preparations of these tumors have demonstrated considerable quantities of an anti-coagulating substance that is presumably heparin (10). The present study demonstrates the pre...

A direct method is presented for the determination of manganese in whole blood or serum by atomic absorption spectroscopy, using a graphite furnace with a temperature ramp controller. Blood or serum is diluted with an equal volume of a five percent solution of Triton-X100 containing heparin. This prevents the blood from coagulating and produces a solution with a viscosity suitable for injection...

The paper presents this new therapeutical method, its. application fields, hypothesis regarding the acting mechanism at the infracellular, cellular and systemic level, the practical methodology, and some personal results. We finish the paper with general proposals for standardization of the scientific works from this field, which should be necessary due to a large variety of approaching to this...

HE SEPARATION of protein from nonprotein material, a usual procedure in the analysis of biological specimens, has been effected in a ilumber of ways. Marymont and London (1) have reported a new coagulalation technic which is perhaps advantageous in certain instances, as well as several applications of the technic (2-6). it is well known that the precipitation of proteins by any of the conventio...

A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG(4)), based on the establishment of N-terminal sequences by automated Edman degradatio...