229 Fecal coliform and Escherichia coli associated with suspended solids (SS) and water in five northern California estuaries were studied to document process influences and water quality monitoring biases affecting indicator bacteria concentrations. We collected and analyzed 2371 samples during 10 sampling events for the five studied estuaries. Concentrations during wet-season stormflow condit...

OBJECTIVES To study bacterial contamination of fresh vegetables before cleaning and before serving to patients in 14 hospitals. MATERIAL AND METHOD Aerobic plate count was performed and emphasized on total viable aerobic bacteria, fecal coliform, fecal Escherichia coli and enteric pathogens in fresh vegetables including romaine lettuce, onion, parsley, celery and tomato before cleaning and be...

The traditional membrane filter (American Public Health Association, Standard Methods for the Examination of Water and Wastewater, 16th ed., American Public Health Association, Washington, D.C., 1985) and presence-absence (P-A) (J. A. Clark, Can. J. Microbiol. 14:13-18, 1968) techniques for the detection of coliform bacteria were compared in a small nonchlorinated drinking water distribution sy...

Many recreational beaches suffer from elevated levels of microorganisms, resulting in beach advisories and closures due to lack of compliance with Environmental Protection Agency guidelines. We conducted the first statewide beach water quality assessment by analyzing decadal records of fecal indicator bacteria (enterococci and fecal coliform) levels at 262 Florida beaches. The objectives were t...

We developed a chemiluminometric assay of beta-galactosidase in coliform bacteria, using a phenylgalactose-substituted 1,2-dioxetane derivative as a substrate. Permeabilization of cells is required to ensure the efficient cellular uptake of this compound. By this method, one coliform seeded in 100 ml of sterile water can be detected after a 6- to 9-h propagation phase followed by a 45-min enzym...

The Honolulu Board of Water Supply (HBWS) selectively chlorinates half of its groundwater supply to provide drinking water that meets the Total Coliform Rule. The objective of this study was to determine whether the groundwater sources used by HBWS are vulnerable to contamination with fecal matter and would require routine disinfection. Larger volumes (1,000 mL) of samples from groundwater and ...

The Science Advisory Panel (SAP) evaluated the potential for microbiological environmental and public health impacts of wastewater discharges from large passenger ships. Historically, fecal coliform bacteria have been used to indicate the presence of sewage in environmental waters and as an index of the sanitary quality of the water. Because fecal coliform are always found in the intestinal tra...