Types I, III, IV, and V collagen were isolated and characterized from eight normal dog hearts and seven with volume-overload hypertrophy. Animals with volume-overload hypertrophy were killed at a time when left ventricular end-diastolic pressure and stiffness were increased. The collagens were characterized by solubility properties, sodium dodecyl sulfate-polyacrylamide gel electrophoresis anal...

X-linked Alport syndrome (AS) is a heritable disorder which is associated with mutations in the type IV collagen oc5(IV) chain gene (COL4A5) located on chromosome X. Following renal transplantation, an average of 6% of male AS patients develop anti-GBM nephritis. We studied the specificity of the antibodies against type IV collagen in the serum of a patient with COL4A5 partial deletion. The spe...

To investigate the anti-fibrotic effects of Astragaloside IV in rats with experimental colitis, and to explore the possible mechanisms, Sprague-Dawley Rats with colitis were induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS). Forty-eight rats were randomly divided into normal group, TNBS model group, SB203580 group and Astragaloside IV group. 150 mg/kg TNBS dissolved in 50% ethanol were gav...

Unilateral posterior crossbite is a malocclusion disease that involves morpho-functional characteristics of masseter muscle; normal or increased activity contralateral muscle and reduced the ipsilateral during unilateral have been shown. Since extracellular matrix plays key role in mechano-transduction transmitting forces contraction, aim present study was to analyse behaviour this type through...

During adipogenic differentiation human mesenchymal stem cells (hMSC) produce collagen type IV. In immunofluorescence staining differentiating hMSCs started to express collagen type IV when Oil Red O-positive fat droplets appeared intracellularly. Quantitative real time-polymerase chain reaction confirmed progressive increase of collagen type IV α1 and α2 mRNA levels over time, 18.6- and 12.2-f...

PURPOSE Collagen gels may prove to be potential carriers for transplantation of cultured corneal epithelial cells. The purpose of this study was to evaluate the suitability of collagen gels in comparison with corneal stromal blocks as the substrate to support the growth of human corneal epithelial cells in culture and the synthesis and deposition of the basement membrane components by these cel...

A novel rat model of nephritis induced by monoclonal antibodies to the non-collagenous domain of type IV collagen was developed by Sado’s group [1-2]. Chondrex, Inc. provides three rat monoclonal antibodies to non-collagenous domain 1 (NC1) of α4 chain of type IV collagen, α4(IV), of rat glomerular basement membrane (GBM), which are used for inducing nephritis in Wistar-Kyoto rats by a single i...

Acute rheumatic fever is a serious autoimmune sequel of Streptococcus pyogenes infection. This study shows that serotype M3 and M18 S. pyogenes isolated during outbreaks of rheumatic fever have the unique capability to bind and aggregate human basement membrane collagen type IV. M3 protein is identified as collagen-binding factor of M3 streptococci, whereas M18 isolates bind collagen through a ...

BACKGROUND Angiotensin II (Ang II) stimulates synthesis of type IV collagen in a cultured murine proximal tubular cell line (MCT cells). In addition, Ang II also induces the expression of TGF-beta1 in these cells. Since TGF-beta has well-known stimulatory effects on the transcription of various collagens, we tested whether the Ang-II-mediated stimulation of type IV collagen is due to induction ...