The hematopoietic stimulatory activities of human recombinant IL-3 and granulocyte-macrophage colony stimulating factor (GM-CSF) were directly compared using highly enriched human bone marrow progenitor target cells. IL-3 supported a larger number of erythroid and megakaryocytic progenitor cells than did GM-CSF, while GM-CSF supported more myeloid progenitors. IL-3 directly stimulated the divis...

Although intracoronary administration of bone marrow–derived mononuclear progenitor cells (BMCs) may be associated with improved cardiac function in patients with chronic postinfarction heart failure, the impact on prognosis and clinical outcome of these patients is unknown. To identify potential predictors for a favorable clinical outcome, we assessed natriuretic peptide serum levels as object...

Although intracoronary administration of bone marrow-derived mononuclear progenitor cells (BMCs) may be associated with improved cardiac function in patients with chronic postinfarction heart failure, the impact on prognosis and clinical outcome of these patients is unknown. To identify potential predictors for a favorable clinical outcome, we assessed natriuretic peptide serum levels as object...

Flt3 is a class 111 tyrosine kinase receptor expressed on primitive human and murine hematopoietic progenitor cells (HPC). In previous studies using stroma-free short term assays, Ftt3 ligand (FL) has been shown to induce proliferation of HPC at proportions similar to or less than c-kit ligand (steel factor, SF). Using long term stromal cocultivation assays, we studied the effects of FL on prol...

BACKGROUND Stethoscope diaphragms have been shown to harbor potentially pathogenic bacteria. OBJECTIVES To assess bacterial contamination on the diaphragm and under the plastic rim that secures the diaphragm of stethoscopes of physicians, nurses, medical students, and house staff in an intensive care unit and a general medical ward of a large university hospital. Also to compare the effective...

OBJECTIVE Although endothelial progenitor cells (EPCs) have been reported to promote neovessel formation during vascular injury, the function of supporting cells of EPCs and their interaction with EPCs during EPC isolation remain unclear. APPROACH AND RESULTS We investigated the functional properties of 2 types of EPCs, also known as endothelial colony-forming cells (ECFCs), CD34(-)/CD34(+) c...

<span lang="EN-US">Colony forming units counting is a conventional process carry out in bacteriological laboratories, and it used to follow the behavior of bacteria different conditions. Currently exist systems, automatic or semi-automatic, colony exits, but, general, many laboratories continue using manual counting, which consumes considerable time effort from researchers laboratory empl...

OBJECTIVE To determine the efficacy of 2.5% and 1.25% Povidone-Iodine solution for Ophthalmia neonatorum prophylaxis. STUDY DESIGN Interventional study. PLACE AND DURATION OF STUDY Eye Department, Combined Military Hospital, Sargodha, from May to November 2014. METHODOLOGY Atotal of 200 eyes of 100 newborn babies were enrolled and divided into two groups of 100 right eyes and 100 left eye...

Previous studies have shown that mouse bone marrow cells can produce mast cells when stimulated in vitro by stem cell factor (SCF) and interleukin-3 (IL-3). Experiments to define the marrow cells able to generate mast cells showed that the most active subpopulations were the Kit(+) Sca1(-) progenitor cell fraction and the more ancestral Kit(+) Sca1(+) blast colony-forming cell fraction. In clon...