Methods: The experiments were performed in a multi-farm system experiencing high nursery mortality (> 4.8%) due to H parasuis infection. In Experiment 1, pigs were vaccinated at weaning using a commercially available, one-dose H parasuis vaccine. In Experiment 2, pigs were vaccinated at processing and at weaning with an autogenous vaccine. In Experiment 3, pigs were orally inoculated at process...

The sulfur cycle of Ebro Delta microbial mats was studied in order to determine sulfide production and sulfide consumption. Vertical distribution of two major functional groups involved in the sulfur cycle, anoxygenic phototrophic bacteria and dissimilatory sulfate-reducing bacteria (SRB), was also studied. The former reached up to 2.2x10(8) cfu cm(-3) sediment in the purple layer, and the latt...

BACKGROUND Bacterial resistance to commonly used antimicrobials is an increasing problem in Asia but information concerning the antimicrobial susceptibility of bacteria causing urinary tract infections (UTIs) in children is limited. METHODS This was a 5-year retrospective study of children with suspected UTI attending a paediatric hospital in north-west Cambodia. Urines with a positive cultur...

Confined animal production systems produce increased bioaerosol concentrations, which are a potential respiratory health risk to individuals on site and downwind. In this longitudinal study, airborne endotoxin and microorganisms were collected during the spring, summer, and fall at a large, open-freestall dairy in southern Idaho. Compared with the background ambient atmosphere, both endotoxin a...

The Guide for the Care and Use of Laboratory Animals states that sanitization of caging accessories (for example, filter tops and wire-bar lids) should be done every 2 wk. In this study we tested the hypothesis that organic contamination measured by the presence of ATP associated with organic material (measured with luciferase test swabs) and the number of bacterial colony-forming units (as det...

The growth requirements of 135 clinical isolates of Neisseria gonorrhoeae and six American Type Culture Collection reference strains were examined by using a simple chemically defined medium known as Wong-Shockley-Johnston medium, WSJM. The simple liquid medium supported growth of gonococci from an inoculum of 2 X 10(6) colony-forming units ml-1 and yielded 10(10) colony-forming units ml-1 in 1...

A review of the literature demonstrated that controversy exists as to whether anesthesia circle systems can be a source of transmission of bacteria to patients. The purpose of this study was to evaluate the use of one airflow filter (on the expiratory limb) versus two airflow filters (one on the inspiratory limb and one on the expiratory limb) in the prevention of bacterial migration in the ane...

A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This com...

A total of 20 patients with symptomatic urinary tract infections (greater than 10(5) colony-forming units of Escherichia coli per ml of urine in addition to pyuria) received 10 mg of ampicillin and 2 liters of fluid daily for 3 days. After 2 days, 16 patients had culture-negative urine without pyuria. A total of 18 similar patients received only 2 liters of fluid for 3 days. On day 4, all had g...