We compared the activities of linezolid (25 mg/kg of body weight, administered intraperitoneally every 8 h) and of vancomycin (25 mg/kg of body weight, administered intraperitoneally every 8 h) in a rat model of vanA vancomycin-resistant Enterococcus faecium experimental endocarditis. Results were expressed as median log(10) CFU per gram of vegetation after 3 days of treatment. The median log(1...

objective: the present study examines the effects of increased heat shock proteins expression in mda-mb468 adeno-carcinoma cells on expansion and differentiation of hematopoetic stem cells from human umbilical cord blood. materials and methods: highly purified human cd34+ cells (magnetic bead isolation) derived from umbilical cord blood were cultured, under three conditions, in the presence of ...

Erythroid progenitors from 1 8 fetal livers (7-1 5-wk gestational period) and 1 3 normal adult marrows were grown in methylcellubose cultures containing erythropoietin (Ep) and burst-enhancing factor(s) (BEF). Additional experiments were carried out on 1 fetal, 1 5 neonatal. and 26 adult blood specimens. Three classes of progenitors (primitive or mature erythroid burst-forming unit(s). P-BFU-E....

The effect of infection with Moloney murine leukemia virus (Mo-MuLV) on long-term bone marrow cultures was studied. Cultures were derived from the bone marrow of BALB/Mo mice, which carry Mo-MuLV as an endogenous virus, or from BALB/c, 129/J, or balb/c X 129/J mice that were infected with Mo-MuLV in vitro. The following parameters were tested: longevity of generation of granulocytes; biological...

There is an antigen on mouse brain tissue which is shared by the hemopoietic colony-forming unit or stem cell of the mouse. Treatment of bone marrow or fetal liver cells with anti-brain antisera inhibits expression of colony-forming units. The anti-stem cell antibody is not absorbed by thymus cells and thus can be distinguished from the anti-thymocyte antibody which these antisera also contain.

OBJECTIVE To determine the number of colony-forming units (CFU) that best correlates with catheter-related infections (CRI) in newborns. METHODS This was a prospective study of semiquantitative cultures of catheter tips obtained from newborns in the neonatal unit at Faculdade de Medicina de Botucatu, state of São Paulo, Brazil. The microorganisms isolated from catheter and peripheral blood cu...

Colonization of the intestinal tracts of newly hatched chicks with Escherichia coli was attempted by swabbing test organisms onto the air-shell of 19-day-old embryos. Test organisms consisted of two virulent E. coli isolates, one avirulent isolate, and one laboratory-derived mutant of the avirulent isolate carrying a recombinant plasmid coding for Colicin V production. Chicks were cultured week...

Biofilm formation and function was studied in mixed culture using 20 bacterial strains isolated from a karst aquifer. When co-cultured in a glucose-limited chemostat, Vogesella indigofera and Pseudomonas putida were the dominant planktonic and biofilm organisms respectively. Biofilm formation and resistance to the iodine disinfectant betadine were then studied with monoculture and binary cultur...

BACKGROUND Effective cleaning of dentures is important to maintain a good oral hygiene for patients suffering from denture stomatitis (DS). This study aimed to evaluate the efficacy of hexetidine 0.1% in eliminating C. albicans colonizing dentures. MATERIALS AND METHODS A total of 40 denture wearers (18 men, 22 women; age range 40-80 years) with clinical evidence of DS were randomly divided i...