Commercial pectolytic enzymes were investigated for their influence on phenolics and antioxidant activities of asparagus juice. The antioxidant activity of asparagus juice was analyzed according to 2,2'-diphenyl-l-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) methods. The enzymes, with the exception of pectinase from Rhizopus sp., contained rutinase, which hydrolyzed ...

REBASE contains comprehensive information about restriction enzymes, DNA methyltransferases and related proteins such as nicking enzymes, specificity subunits and control proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data. Homing endonucleases are also included. REBASE contains the most co...

Fermented food provides plenty of vital nutrients and bioactive components that affect a number of functions of human body in a positive way. Fermented dairy and food products can be made more functional by incorporating probiotic bacteria such as strains belonging to genera Lactobacillus and Bifidobacterium. A few strains of such food grade bacteria are capable of synthesizing essential biomol...

Flavonoid-rich extract prepared from Glycyrrhiza glabra has been found to be beneficial in patients with functional dyspepsia and was reported to possess some gut health-promoting properties such as antioxidant, anti-inflammatory and anti-Helicobacter pylori activities. In the present study, the flavonoid-rich extract of Glycyrrhiza glabra was evaluated for its compatibility with probiotic stra...

Proteases are catabolic enzymes that catalyze the complete hydrolysis of protein. They constitute one of the most important groups of industrial enzymes, accounting for nearly 60% of the total worldwide enzyme sale1. Keratinolytic protease is a specific protease that has immense commercial importance. It acts on keratin of the hides thus can be used in dehairing2. This enzyme along with proteas...

Abstract Hydrothermal methods are commonly applied in pretreatment of lignocellulose for conversion to biofuels and chemicals. This partially disassembles solubilizes hemicelluloses lignin, releasing sugars phenolic compounds, which potential inhibitors the subsequent enzymatic saccharification step. In this work, inhibitory effect soluble phenolics hydrothermal liquor on canonical Trichoderma ...

To check the suitability of enzyme entrapped beads for use in pharmaceutical industry, amylase enzyme was entrapped in agar/agarose, polyacrylamide gels and calcium alginate beads. Sodium alginate of 1% concentration was found to be best with respect to immobilization efficiency and calcium alginate beads so obtained were not much susceptible to breakage. When sodium alginate- amylase mixture w...

Worldwide mycotoxins contamination has a significant impact on animal and human health, and leads to economic losses accounted for billions of dollars annually. Since the application of pre- and post- harvest strategies, including chemical or physical removal, are not sufficiently effective, biological transformation is considered the most promising yet challenging approach to reduce mycotoxins...

This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R,S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the conversion will reduce the mass of material that requires racemisation and recycling. Hence the aim was to find an enzyme that yields (S)-naproxen with an ena...

The objective of the present study was twofold: first to compare the degradation patterns of caprine whey proteins digested with either human digestive juices (gastric or duodenal) or commercial porcine enzymes (pepsin or pancreatic enzymes) and second to observe the effect of gastric pH on digestion. An in vitro two-step assay was performed at 37 degrees C to simulate digestion in the stomach ...