Soluble components of type 8 adenovirus have been studied. Four different components were isolated by anion-exchange chromatography and purified by further chromatographic procedures, by zonal centrifugation, and by erythrocyte absorption and elution. The four components exhibited the following characteristics. (i) Fiber antigen was trypsin-resistant and functioned as incomplete hemagglutinin (...

We performed a comparatÍve study bebween xenodiagnosis and serological tests for Chagasfdisease. 150 Patients from several endemic areas were studied. Four of them appeared to have a peculiar status with positive xenodiagnosis and negative serclogy carr'ied out with four classical techniques (Immunofluorescence test, ELrsA: Enzyme Linked rmmunosorbent Assay, complement fixation test and Imrnuno...

Introduction The year 2006 represented the centenary of the first diagnostic serological test, the Wasserman complement fixation test for the diagnosis of syphilis. In this article I will relate some developments I have observed in the day-to-day running of a diagnostic serology laboratory over the thirty years preceding this centenary. I have assumed some background knowledge and elected to di...

Complement-fixation and radioactive-agglutination tests were performed on purified preparations of adenovirus type 5 using specific antisera against capsid antigens and an antiserum which can detect an antigen (the P antigen) in infected cells which has many of the properties of the T antigen associated with infection by the oncogenic adenoviruscs. A serological reaction was detected between pr...

The Serodia Myco II particle agglutination test, which the manufacturers claim exclusively detects IgM antibody, was compared with two IgM-specific tests, a mu-capture ELISA, and indirect immunofluorescence for their ability to detect recent Mycoplasma pneumoniae infection. In general there was good agreement among the three tests, all three having similar sensitivity. One hundred and nine (78%...

The technique of complement fixation test as a measure for the serological diagnosis of neurotropic virus infections was first put in practice by Casals and Palacios (1) in the year 1941, and confirmed of its practical diagnostic value by numerous follow-ups made after it. In spite of repeated attempts, the application of this technique for the diagnosis of poliomyelitis had not been successful...

Passive protection of mice against Pseudomonas aeruginosa using specific antisera and immunoglobulin fractions induced by immunizing rabbits with a ribosomal vaccine is reported. The results demonstrated that protection by the ribosomal vaccine against challenge with live organisms can be serum mediated. Previous work has shown that the vaccine can be separated into two components on the basis ...