In vitro selections for catalytic activity have been designed for the isolation of genes encoding enzymes from libraries of proteins displayed on filamentous phages. The proteins are generally expressed as C-terminal fusions with the N-terminus of the minor coat protein p3 for display on phages. As full-length cDNAs generally contain several stop codons near their 3' end, this approach cannot b...

Oligonucleotide and cDNA microarrays are highthroughput techniques that compare the mRNA expression of a large group of genes in different cells or experimental conditions. The final output of a microarray data analysis is a list of codes identifying differentially expressed transcripts. Interactions among genes, however, are not adequately represented by a list of codes. Therefore, the followi...

We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3' ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5' ends of the inserts to che...

BACKGROUND The skin has many important functions such as protection, preservation, temperature regulation, and vitamin D synthesis. It is composed of a variety of cell types including keratinocytes, melanocytes and fibroblasts. OBJECTIVE We attempted to compare the gene expression profiles between keratinocytes, melanocytes and fibroblast, using cDNA microarray. METHODS Keratinocytes, melan...

Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. T...

We have conducted a sequencing project of human cDNAs which encode large proteins in brain. For selection of cDNA clones to be sequenced in this project, cDNA clones have been experimentally examined by in vitro transcription/translation prior to sequencing. In this study, we tested an alternative approach for picking up cDNA clones having a high probability of carrying protein coding region. T...

Xenopus laevis short interspersed repeat transcripts (Xlsirts) are a family of noncoding RNAs defined by the presence of a specific repeated sequence that acts as a vegetal localization element. Previous studies have demonstrated that Xlsirts function as localization elements to localize RNA and also in anchoring mRNA at the vegetal cortex. However, the identity of the Xlsirts containing family...

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with signific...

A complementary DNA (564 base pairs) encoding for the mature region of the growth hormone (GH) of yellowfin porgy Acanthopagrus latus was amplified by polymerase chain reaction. This resultant DNA fragment , ypmGH cDNA, was inserted into baculovirus expression vectors, pAc7 , under the control of the polyhedrin promoter. A 22 kDa pro tein, similar to that of authentic GH , was expressed intrace...

Two zygotic genes, twist and snail, are indispensable for the correct establishment of the mesoderm primordium in the early Drosophila embryo. They are also needed for morphogenesis and differentiation of the mesoderm. Both genes code for transcription factors with different, albeit complementary, functions. Therefore, to understand the early development of the mesoderm, it will be necessary to...