WE-14 is generated in subpopulations of chromogranin A immunopositive endocrine cells and neurons including those innervating the anterior uvea. This study investigated WE-14 in intact sclero-limbo-corneal tissue from embryonic (E17), neonatal (N0-N16), and adult mice using immunocytochemistry and confocal scanning laser microscopy. Weak WE-14 immunostaining was observed at birth in nerve fibre...

This study compared the abnormal corneal epithelium after laser-assisted subepithelial keratectomy (LASEK) with dystrophic cornea using in vivo using confocal microscopy (IVCM) and examined the effects of the abnormal epithelium on postoperative recovery of uncorrected distance visual acuity (UDVA) and sub-basal nerve plexus regeneration. The UDVA and wound healing were examined in 50 eyes of 2...

PURPOSES To describe corneal changes seen on in vivo confocal microscopy in patients with total limbal stem cell deficiency and to correlate them with cytological findings. METHODS A prospective case series including 13 eyes (8 patients) with total limbal deficiency was carried out. Stem cell deficiency was diagnosed clinically and by corneal impression cytology. Confocal images of the centra...

The presence of heme oxygenase and NADPH cytochrome P-450 (c) reductase, the latter an integral component of heme oxygenase and cytochrome P-450-dependent drug metabolizing enzymes, was demonstrated in human corneal epithelium. We reported for the first time that human corneal epithelium contains heme oxygenase activity as high as 20% of that reported for the human liver. Using immunological te...

hydrargyri perchloridum solution and later with sterile distilled water. Saline is not to be used on account of its decomposing effects on the solutions used for tattooing. The next step is to scrape off the superficial corneal epithelium. Ordinary Graefe's knives, secondary knives, scalpels, discission needles, pterygium knives and many other instruments have been used to scrape the cornea, bu...

The corneas of albino rabbits were irradiated (5 min exposure once a day) with UVB rays (312 nm) for 4 days (shorter procedure) or 8 days (longer procedure). The eyes were examined microbiologically and only the corneas of sterile eyes or eyes with non-pathogenic microbes were employed. Histochemically, the activities of reactive oxygen species (ROS)-generating oxidases (xanthine oxidase, D-ami...

Measurements of oxygen profiles across the cornea, aqueous humor, and anterior lens differed from previously calculated values. The aqueous humor oxygen tension in paiticular also differed from previously measured values, being approximately 20 mm. Hg higher. Reasons for these differences are discussed. The tissue oxygen gradients measured confirm the high Qos of lenticular and corneal epitheli...

The embryonic chick corneal epithelium produces a highly structured acellular matrix beneath its basal surface during early development. This matrix, which contains collagen, serves as a morphogenetic template for subsequent stromal development in that the three-dimensional architecture of the adult corneal stroma is initially established, in miniature, in this epithelially derived connective t...

PURPOSE To develop an in vitro model of human corneal epithelium that can be propagated in serum-free medium that is tissue specific, species specific, and continuously available. METHODS Primary explant cultures from human cadaver donor corneas were generated and subsequently infected with Adeno 12-SV40 (Ad12-SV40) hybrid virus or transfected with plasmid RSV-T. RESULTS Several lines of hu...