نتایج جستجو برای: covalently attachment

تعداد نتایج: 55663  

Journal: :Antimicrobial agents and chemotherapy 2010
Manuela Donalisio Marco Rusnati Andrea Civra Antonella Bugatti Donatella Allemand Giovanna Pirri Andrea Giuliani Santo Landolfo David Lembo

Peptide dendrimers consist of a peptidyl branching core and/or covalently attached surface functional units. They show a variety of biological properties, including antiviral activity. In this study, a minilibrary of linear, dimeric, and dendrimeric peptides containing clusters of basic amino acids was evaluated for in vitro activity against human papillomaviruses (HPVs). The peptide dendrimer ...

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2001
J J Gooding P Erokhin D Losic W Yang V Policarpio J Liu F M Ho M Situmorang D B Hibbert J G Shapter

The fabrication of enzyme electrodes using self-assembled monolayers (SAMs) has attracted considerable interest because of the spatial control over the enzyme immobilization. A model system of glucose oxidase covalently bound to a gold electrode modified with a SAM of 3-mercaptopropionic acid was investigated with regard to the effect of fabrication variables such as the surface topography of t...

Journal: :Lab on a chip 2016
M Parvinzadeh Gashti J Asselin J Barbeau D Boudreau J Greener

A microfluidic platform with a fluorescent nanoparticle-based sensor is demonstrated for real-time, ratiometric pH imaging of biofilms. Sensing is accomplished by a thin patterned layer of covalently bonded Ag@SiO2+FiTC nanoparticles on an embedded planar glass substrate. The system is designed to be sensitive, responsive and give sufficient spatial resolution to enable new micro-scale studies ...

Journal: :Analytical chemistry 2006
Jhony Orbulescu Celeste A Constantine Vipin K Rastogi Saumil S Shah Joseph J Defrank Roger M Leblanc

As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...

Journal: :Cancer research 1987
K P Borlinghaus D A Fitzpatrick N D Heindel J A Mattis B A Mease K J Schray D J Shealy H L Walton D V Woo

Misonidazole was covalently conjugated (3-68 mol drug/mol antibody) to 19-9 monoclonal antibody directed against a colorectal carcinoma tumor-associated antigen as a method for targeting radiosensitizing agents. This attachment was accomplished by the mixed anhydride method using the hemisuccinate derivative of misonidazole. Evaluation of conjugates in vitro shows a loss of antibody binding act...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1979
O C Richards E Ehrenfeld J Manning

Poliovirus-specific double-stranded RNA molecules containing covalently attached protein were coupled with a biotin ester through the protein moiety. Subsequent interaction of the RNA-biotin with avidin attached to electronopaque plastic spheres led to the formation of complexes that were easily visualized in the electron microscope. Avidinspheres were associated only with one end of the RNA-bi...

Journal: :Journal of lipid research 2009
Robert C Murphy Joseph A Hankin Robert M Barkley

Recent developments in MALDI have enabled direct detection of lipids as intact molecular species present within cellular membranes. Abundant lipid-related ions are produced from the direct analysis of thin tissue slices when sequential spectra are acquired across a tissue surface that has been coated with a MALDI matrix. The lipid-derived ions can often be distinguished from other biomolecules ...

Journal: :Chemphyschem : a European journal of chemical physics and physical chemistry 2014
Wendu Ding Christian F A Negre Julio L Palma Alec C Durrell Laura J Allen Karin J Young Rebecca L Milot Charles A Schmuttenmaer Gary W Brudvig Robert H Crabtree Victor S Batista

Linkers that favor rectification of interfacial electron transfer are likely to be required for efficient photo-driven catalysis of multi-electron reactions at electrode surfaces. Design principles are discussed, together with the synthesis and characterization of a specific pair of molecular linkers, related by inversion of the direction of an amide bond in the heart of the molecule. The linke...

Journal: :Nucleic acids research 1985
H P Hershey R F Barker K B Idler J L Lissemore P H Quail

Cloned cDNA and genomic sequences have been analyzed to deduce the amino acid sequence of phytochrome from etiolated Avena. Restriction endonuclease site polymorphism between clones indicates that at least four phytochrome genes are expressed in this tissue. Sequence analysis of two complete and one partial coding region shows approximately 98% homology at both the nucleotide and amino acid lev...

Journal: :The Biochemical journal 1978
C Jacobsen

Bilirubin can be coupled covalently to albumin by using water-soluble carbodi-imide as coupling reagent. The optimal specificity in the attachment of bilirubin to the high-affinity site on the albumin molecule was obtained by treating an albumin-bilirubin complex with carbodi-imide in low concentrations and for a short period. The product was reduced, carboxymethylated and digested with trypsin...

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