BACKGROUND We investigated whether the endoplasmic reticulum (ER) stress response could be a cyclooxygenase-2 (COX2)-independent mechanism of growth inhibition by celecoxib in a head and neck squamous cell carcinoma (HNSCC) cell line. MATERIALS AND METHODS We performed western blotting and reverse transcription polymerase chain reaction to analyze the expression of ER stress response-associat...

We have further defined mechanism(s) by which 2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl}acetamide [OSU-03012 (OSU)], a derivative of the cyclooxygenase-2 (COX2) inhibitor celecoxib but lacking COX2 inhibitory activity, kills transformed cells. In cells lacking expression of protein kinase R-like endoplasmic reticulum kinase (PERK(-/-)), the lethality of OSU w...

NFAT (nuclear factors of activated T cells) proteins constitute a family of transcription factors involved in mediating signal transduction. The presence of NFAT isoforms has been described in all cell types of the immune system, with the exception of neutrophils. In the present work we report for the first time the expression in human neutrophils of NFAT2 mRNA and protein. We also report that ...

The high fat content in Western diets probably affects placental function during pregnancy with potential consequences for the offspring in the short and long term. The aim of the present study was to compare genome-wide placental gene expression between rat dams fed a high-fat diet (HFD) and those fed a control diet for 3 weeks before conception and during gestation. Gene expression was measur...

Two series of chalcone/aryl carboximidamide hybrids 4a–f and 6a–f were synthesised evaluated for their inhibitory activity against iNOS PGE2. The most potent derivatives further checked in vivo anti-inflammatory utilising carrageenan-induced rat paw oedema model. Compounds 4c, 4d, 6c 6d proved to be the effective inhibitors PGE2, LPS-induced NO production, activity. Moreover, showed significant...

In the present study, complete mitochondrial genomes of five Peltigera species (Peltigera elisabethae, neocanina, canina, ponojensis, neckeri) were sequenced, assembled and compared with relative species. The mitogenomes all composed circular DNA molecules, their ranged from 58,132 bp to 69,325 bp. contain 15 protein-coding genes (PCGs), 2 rRNAs, 26–27 tRNAs an unidentified open reading frame (...

Background: Jasminum grandiflorum is a medical plant widely used in Ayurvedic herbal medicines India. This host to very novel medicinal potential molecules such as iridoids. Flavonoids and other small are also elaborated by this plant. The present study was aimed understand the computational inhibition of phytochemicals against anti-inflammatory molecular targets. Methods: iridoids, flavonoids ...

Kawasaki disease is an acute illness of early childhood that is characterized by prolonged fever and vasculitis of unknown pathogenesis. Lactobacillus casei cell wall extract (LCWE)-induced vasculitis in mice is a well-validated model of Kawasaki disease. In the nervous system, glial cells play an important role in fever development. This study investigated whether LCWE directly stimulates glia...

The high fat content in Western diets probably affects placental function during pregnancy with potential consequences for the offspring in the short and long term. The aim of the present study was to compare genome-wide placental gene expression between rat dams fed a high-fat diet (HFD) and those fed a control diet for 3 weeks before conception and during gestation. Gene expression was measur...