A cascade recycling amplification (CRA) that implements cascade logic circuits with feedback amplification function is developed for label-free chemiluminescence detection of microRNA-122 with an ultrahigh sensitivity of 0.82 fM and excellent specificity, which is applied to construct a series of molecular-scale two-input logic gates by using microRNAs as inputs and CRA products as outputs.

BACKGROUND Implementation support models are increasingly being used to enhance the delivery of evidence-based treatments (EBTs) in routine care settings. Little is known about the extent to which these models lead to continued EBT use after implementation support ends. Moreover, few empirical studies longitudinally examine the hypothesized factors associated with long-term psychosocial EBT use...

BACKGROUND E. coli B (BL21), unlike E.coli K-12 (JM109) is insensitive to glucose concentration and, therefore, grows faster and produces less acetate than E. coli K-12, especially when growing to high cell densities at high glucose concentration. By performing genomic analysis, it was demonstrated that the cause of this difference in sensitivity to the glucose concentration is the result of th...

Optical network uses a tool for routing called Latin router. These routers use particular algorithms for routing. For example, we can refer to LDF algorithm that uses backtracking (one of CSP methods) for problem solving. In this paper, we proposed new approached for completion routing table (DRA&CRA algorithm) and compare with pervious proposed ways and showed numbers of backtracking, blocking...

S. Jacob, E. Vangdal, A. Torricelli, L. Spinelli, M. Vanoli, P. Eccher Zerbini, L.M.M. Tijskens and E. Madieta 1 Laboratoire GRAPPE, Groupe-ESA, Angers, France 2 CRA-IAA, Unità di ricerca per i processi dell’industria agroalimentare (Agricultural Research Council Research Unit for Agro Food Industry formerly CRA-IVTPA), Italy 3 Bioforsk Vest Ullensvang, Lofthus, Norway 4 CNR-INFM and CNR-IFN, P...

In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c mice immunized with cytoplasmic repetitive antigen (CRA), and flagellar repetitive antigen (FRA) of Trypanosoma cruzi. The immunization was done by subcutaneous route three times (20 days apart) and the analysis was performed 14 days after each treatment. CRA-immunized mice produced high levels of a...

32 The rising costs of complying with supervisory demands have brought the issue of regulatory burden to the attention of both lawmakers and bank regulators. But one relatively underappreciated aspect of regulatory burden is the potential for the supervisory process to impose conflicting demands on banks. In October 1977, Congress passed the Community Reinvestment Act (CRA) as Title VIII of the...