A method is described for enzymatic measurement of serum creatinine using a centrifugal analyzer. The method employs fixed-time kinetics and a true serum blank. The method required 100 microliters of serum and is linear to 90 mg per L. Day-to-day precision studies revealed a standard deviation (coefficient of variation) of less than +/- 0.5 mg per L (7.4 percent) at a mean creatinine concentrat...

A similar expression would result if different dilution steps were used for each ampoule or different coefficients of variation were used for different dilution steps or solutions. The sum above (S2) would include terms (1 d)2(Cv2 + CA2) with appropriate values of d, Cv and CA for each step. If R is the estimate of the relative potency computed for an assay, then for known relative potency, r, ...

Background: Microalbuminuria is a microvascular complication of diabetes mellitus. Physical exercise has effects on urinaryalbumin-creatinine ratio and blood pressure in type 2 diabetic male with microalbuminuria.Objective: To assess the physical urinary albumin-creatinine microalbuminuria.Materials Methods: This prospective interventional study was carried out Department Physiology, Sir Salimu...

(/32-M) involving a commercially available monoclonal antibody, we used a second monoclonal antibody, produced in our laboratory, for affinity-chromatographic purification of /32-M. Both monoclonal antibodies bound to all of the charge isomers of f32-M identified by two-dimensional gel electrophoresis. Polyethylene glycol was used to separate the phases after incubation for 3 h at room temperat...

Agricultural workers are in danger of being exposed to toxic substances such as pesticides. To estimate the individual handling pesticides, bio-monitoring effects on agricultural is required. There has been no research previously conducted among Libyan workers. This was designed study biochemical parameters impacts pesticide contamination at Aljebal Alakhtar, Libya. 45 blood samples were taken ...

BACKGROUND The nephrotoxicity induced by contrast media remains a serious clinical problem, and the underlying mechanism has not been completely understood. Experimental and clinical investigations suggest that reactive oxygen species (ROS) are critical determinants of radiocontrast nephropathy (RCN), and that antioxidants can prevent this damage. METHODS Cultured human proximal renal tubule ...

and 2.0% for patients’ samples having mean total CO2 of 7 and 35 mmol/L, respectively (n = 27). Least-squares linear-regression analysis of the total CO2 values obtained from the potentiometric (x) and new enzymatic slides (y) for 113 clinical samples (range 15-41 mmol/L) showed the following relationship: y = 0.92 x + 0.35 (r = 0.976, P <0.01) (4). Only samples assayed within 5 miii of each ot...