نتایج جستجو برای: cross linking
تعداد نتایج: 536744 فیلتر نتایج به سال:
Glutaraldehyde possesses unique characteristics that render it one of the most effective protein crosslinking reagents. It can be present in at least 13 different forms depending on solution conditions such as pH, concentration, temperature, etc. Substantial literature is found concerning the use of glutaraldehyde for protein immobilization, yet there is no agreement about the main reactive spe...
A novel bifunctional thioimidate cross-linking reagent (diethyl suberthioimidate) that modifies amines without sacrificing their native basicity is developed. Intermolecular cross-linking of neurotensin and intramolecular cross-linking of cytochrome c under physiological conditions is investigated with this reagent. Because it does not perturb the electrostatic properties of a protein, it is un...
Chemical cross-linking of protein complexes has gained renewed interest in combination with mass spectrometric analysis of the reaction products as it allows a rapid mapping of protein interfaces, which is crucial for understanding protein/protein interactions. The identification of cross-linking products from the complex mixtures created after the cross-linking reaction, however, remains a dau...
The capsid of bacteriophage HK97 is stabilized by approximately 400 covalent cross-links between subunits which form without any action by external enzymes or cofactors. Cross-linking only occurs in fully assembled particles after large-scale structural changes bring together side chains from three subunits at each cross-linking site. Isopeptide cross-links form between asparagine and lysine si...
In the present study, the effect of stirring speed and the type of cross linking agent on the size and formation of microencapsulated Silver carp (Hypophthalmichthys molitrix) oil were investigated. The gelatin/gum Arabic was used for encapsulating and the capsules were prepared by complex coacervation. Microcapsules were analyzed by optical microscopy technique and particle size analyzer. Re...
Hydrogels are introduced to modem medicine as novel materials suitable for a variety ofbiomedical applications. Studying hydrogels as novel biomaterials has become a fast-developingand exciting research field during the last two decades. These interesting biomaterials have found awide range of application including contact lenses, vehicles for drug delivery and scaffold in tissueengineering and...
To the Editor: The same stromal opacities found in the rabbit study by Salomão et al, 1 which appeared in the June 2011 issue of the Journal of Refractive Surgery, have been previously described as frequent but asymptom-atic complications after corneal cross-linking (CXL) in cases of steep corneas with keratometry values Ͼ54.00 diopters (D). 2-4 Raiskup et al 4 demonstrated that infusion with a...
The management of keratoconus and post – LASIK ectasia included until recently spectacle correction, Hard Fitted Gas Permeable Contact Lenses, Intrastromal Corneal Ring Segments. All these interventions are purely symptomatic and their only aim is the improvement of visual function of patients, without interfering with the pathophysiology of the ectatic disorders. The only actual treatment for ...
Cross-linked bioreducible layer-by-layer films for increased cell adhesion and transgene expression.
The effect of cross-linking layer-by-layer (LbL) films consisting of bioreducible poly(2-dimethylaminoethyl methacrylate) (rPDMAEMA) and DNA is examined with regard to rigidity, biodegradability, cell adhesion, and transfection activity using 1,5-diiodopentane (DIP) cross-linker. DIP chemically reacts with the tertiary amines of rPDMAEMA, altering the chemical composition of these LbL films. Th...
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